THE OLIVE tubercle: CAUSE 403 



sky's solution containing 3 per cent xylose and the fluid be- 

 comes acid (Petri). We could not get this result with our xylose. 

 Growth in Winogradsky's solution (nitrogen-free medium) with 

 3 per cent glucose, arabinose or xylose, is good, but in the same 

 with 3 per cent saccharose, lactose or mannit, is scarcely appre- 

 ciable (Petri). We could not verify these statements. A culture 

 20 days old in Dunham's solution, when tested for indol, gave 

 a reddish color (Petri). Not much indol is produced (Smith, 

 Petri). Confirmed by Miss Brown in 1915. Slow-growing, 

 white colonies appeared on 1 per cent grape-sugar or cane- 

 sugar agar (1.5 per cent agar) reinforced with a neutralized 

 decoction of young olive shoots, the tannin compounds inter- 



FiG. 31L — Bacterium savastanoi. Surface colony on +10 beef-peptone gelatin 

 37 days at 16°C. Photographed in 1908. X 5. 



fering with development (Petri). No growth for us. It is 

 viscid on cooked potato (Petri). The most rapid and abundant 

 growth was in Cohn's solution plus 1 per cent anhydrous dex- 

 trose, in which there was an abundant white precipitate with 

 formation of a thick pellicle and after about 20 days a pale 

 greenish color (pea-green) in the upper part of the fluid (Petri). 

 Correct, except that we could not verify the greenish color with 

 the Portofino olive organism (80 days), but did so with the 

 Vienna ash organism (28 days and later). Neutral bean agar 

 is said to be a good medium for long-continued growth, 

 especially when a trace of sodium phosphate or potassium 



