miscellaneous: subjects for special study 475 



3. Carbon and nitrogen nutrition of the soft-rot organisms. 

 Species relations. Toleration of acids and alkalies. 



4. Carbon and nitrogen nutrition of the yellow Bacterium 

 (Pseudomonas) group. Species relations. 



5. Field studies of soil relations of Bacterium solanacearum, 

 especially to lime, phosphates and potash. 



6. Climatic studies of Bacterium solanaceanmi. Determina- 

 tion of its northern and western extension in the United States. 



7. Geographical distribution of Bacterium solanacearum 

 in Europe and in South America. Ditto Africa and Asia. 



8. Exact determination of the causes of the bacterial potato 

 rots of Australia and New Zealand — of France and Italy. 



9. Comparative studies of the white organisms Bacillus 

 tracheiphilus, Bacillus amylovorus, Bacterium andropogoni, Bac- 

 terium woodsii, and Bacterium mori. 



10. Does Bacterium andropogoni attack maize as well as 

 broom corn and sorghum? 



11. Comparison of the above with Bacillus coli, Bacillus 

 typhosus, and other related animal pathogenes. 



12. Comparison of the soft-rot bacteria with B. lactis, B. 

 coli, etc., in all their varieties, 



13. Comparative study of the green fluorescent pathogenic 

 species. 



14. Studies of sub-species of various pathogenes. There are 

 a good many. 



15. Critical study of the chemistry of all the tumor-produc- 

 ing-species, Bad. tumefaciens in its varieties, Bact. savastanoi, 

 Bad. heticolmn, etc., determinations to be made from young, 

 middle-aged and old flask-cultures in various media. 



16. Determination of all acids produced by plant pathogens. 



17. Hydrogen-ion content of media as related to growth of 

 plant pathogenic species. Conversion of Fuller's scale and 

 Clark's scale to PH. 



18. Adaptation of the newer culture media used by animal 

 pathologists to plant bacteriology. 



19. New differential media for separating closely related 

 forms, such as the various soft-rot organisms and the members of 

 the yellow Bacterium (Pseudomonas) group. 



