400 ODOROGKAPHIA. 



chloroform at once gave rise to the exceedingly disagreeable 

 characteristic odour of iso-nitrile. The indo-phenol reaction took 

 place very beautifully with hydrochloric acid, carbolic acid, 

 solution of chlorinated lime and ammonia, and after treatment 

 with hvdrochloric acid the addition of freshly prepared chlorine 

 water produced a fine bright- blue coloration, which disappeared 

 after some time. All these reactions pointed to the probability 

 that the body under treatment was acetanilide. The correctness 

 of this supposition was further confirmed Ijy analysis and nitrogen- 

 determination. 



" In order to make doubly sure, the bromine combination of the 

 body was formed by dissolving it in glacial acetic acid, and 

 saturating with bromine. The crystals separating after a short 

 time from the acetic acid solution were re-crystallised from alcohol. 

 Their melting point lay near 165^, and the crystals otherwise 

 behaved exactly as does the bromine combination of acetanilide 

 (paracetic bromine-anilide), of which the melting point is 165^. 



" These examinations had made it clear that the artificial musk 

 consists of two bodies, an odorous one, which is present in small 

 proportions (probably tri-nitrobutyl-toluol), and an inodorous one, 

 consisting of acetanilide (" antifebrin "). It now became a matter 

 of interest to determine, at any rate approximately, the percentage 

 proportion of odorous substance in the original preparation. Un- 

 fortunately the petroleum-ether process was not available for this 

 purpose, acetanilide being sufficiently (though only slightly) soluble 

 in petroleum-ether to influence the result too powerfully, consider- 

 ing the small proportion of odorous matter. On this account the 

 determination of the nitro-groups according to Limprichtf as 

 modified by Spindler:|: was resorted to. Although as recorded by 



* Nitrogen estimation by Dumas' method. 

 t Ber. Deutscli. Cliem. Ges., ii., p. 36. 

 X Liel)ig's Annalen, ccxv., p. 288. 



