216 TRANSACTIONS OF THE CANADIAN INSTITUTE. [VoL. 11. 



is irrei^ularly marked with slight depressions which cross it at right angles 

 to the long diameter of the growth. In many places the growth looks 

 like drops of yellowish wax on the potato. The color of the potato does 

 not seem to be altered by the growth. 



Temperature. — Grows at the temperature of the room, and it seems to 

 be very susceptible to heat, the warmth necessary for keeping the agar- 

 agar tubes fluid seemingly destroying it. 



Spore formation. — 



Color. — Not any, except slight tinge of yellow in the potato growth. 



Action on gelatine. — Grows fairly rapidly and is a slow liquefier through- 

 out — lo cc. of gelatine not being all liquefied in 33 days. 



Bacillus B occurred only in 7 per cent, of the tubes. Under the 

 microscope it appears as a very large bacillus, rounded ends and showing 

 tendency to go in pairs and chains of three or four. Very marked spore 

 formation. Size, v^ji X Q'O/j'. Stains well with all the stains and with 

 Gram's, Carbolic Fuchsin, etc. 



Hanging drop. — Motionless. 



Culture. — Appeared in the original tube as white growth spreading 

 much on surface and not elevated. 



Agar-agar plate. — Oval, yellowish white, large colony. Under low 

 power — oval, blackish, thick colony, very granular, dome shaped, edges 

 very rough and the colony irregular in outline. 



Agar-agar stab. — Rapid growth, spreads much on the surface as 

 slightly elevated, smooth, glistening, white colony with somewhat irregu- 

 lar outline. Grows well in the substance of the agar-agar along the 

 track of the needle, but tapering away from the surface. After three 

 weeks the growth becomes yellowish tinted. 



Gelatine plate. — After three days, the deep colonies appear as small, 

 round, white dots and those on the surface as irregularly round, white, 

 and spreading to about i m.m. in diameter. Under low power the 

 surface colonies appear as very irregularly round, yellowish brown, 

 mottled, flat, opaque colonies, slightly thicker in the centre, with very 

 irregularly indented outline, the projections not being sharp but all round- 

 ed at points, and it is seen that this is due to the fact that the colony is 

 made up of a mass of fine intertwining threads and these do not project 

 singly but curve around and give the consequent appearance to the 

 projections. The colony is very suggestive of the " caput medusae." 

 The deep colonies are small round, opaque, flat growths with fine barb- 



