1890-91.] PEACH YELLOWS. 217 



like projections from the edge. After five days liquefaction first begin- 

 ning, being shown by a depressed zone of clear gelatine. 



Gelatine stab. — An exceedingly slow liquefying growth, and only lique- 

 fies around the needle puncture, at the entrance to gelatine, after 15 

 days. Grows very slowly in the gelatine along the needle track as a 

 yellowish white, finely granular, tapering growth, not spreading in the 

 substance of the gelatine. It spreads on the surface slightly and appears 

 as a yellowish white, smooth, slightly elevated, moist looking colony 

 with irregular outline. The liquefaction proceeds very slowly and in the 

 same manner as Bacillus A {i.e., in champagne-glass form), the surface 

 of the fluidified gelatine being covered by the original surface growth 

 and the liquefied gelatine opaque from finely suspended white granules 

 There is a yellowish while granular precipitate at the bottom of the 

 liquefied gelatine. Rest of the medium is quite clear and solid. After 

 a time the surface colony subsides, and the liquefaction has extended to 

 margins of the gelatine. 



BouilLn — Does not m ike it cloudy, but grows up the side of the tube 

 from the bottom, appearing as very finely granular. No growth on the 

 surface, but slight precipitate. 



Potato. — Rapid growth, yellowish, abundant, spreading over the sur- 

 face of the potato, slightly elevated, edges smooth, but irregular contour, 

 surface moist, glistening and smooth. After two weeks the growth 

 becomes markedly yellow and is thrown into numerous folds. 



Temperature.— Gro\ws at ordinary room temperature ; not susceptible 

 to heat as Bacillus A. 



Spore formation. — Very marked ; sometimes three or four large, oval 

 spores seen lying irregularly within a bacillus. 



Color. — Yellowish tinge to growth on both agar-agar and gelatine, and 

 a marked yellow growth on potato. 



Action on gelatine. — Grows slowly in gelatine, and is a slow liquefier. 



Bacillus C occurs also in 7 per cent, of the tubes. Under the micro- 

 scope it appears as a long, thick bacillus with square ends and corners 

 rounded off. Spores noticed — occurs much in pairs and long chains. 

 Size, "9 to I'O iJ- X 27 to y6 P: 



Hanging drop. — Slight movement. 



Culture. — In original tube appeared as a thin, white growth, spread over 

 the lower portion of the agar-agar. 



Agar-agar plate. — White, oval, fairly large colony. Under low power. 



