218 TRANSACTIONS OF THE CANADIAN INSTITUTE. [YoL. II. 



dirty whitish yellow, granular, thick, irregularly oval colony with indented 

 edges. Dome shaped. 



Agar-agar stab. — Very slow growth along the track of the needle 

 when it appears as a well marked, white, rough-edged growth, same 

 thickness along whole length of puncture. Does not grow on the 

 surface until after a week, when it appears as very irregular, slightly 

 elevated, thin, whitish, smooth surfaced growth, and not spreading but 

 very little on the surface of the agar-agar. 



Gelatine plate. — Colonies develop slowly and appear after five or six 

 days. Those in the depth are small, round and white. Superficial ones 

 are thin, white and more spreading. After six days a small depression 

 of commencing liquefaction seen which then continues quite rapidly. 

 Under low power the superficial ones appear as flat, slightly darker in 

 centre, yellowish colonies with very irregular outline, and the whole 

 growth seen to consist of fine threads, twisted in all directions and pro- 

 jecting into the gelatine and giving the colony a hairy appearance. The 

 deep colonies are dark brownish yellow with opaque centre being slightly 

 thinner at margin. Some small projecting fibrillae, which do not seem 

 to extend into liquefying zone. 



Gelatine stab. — Very slow growth and still slower liquefaction. At 

 first grows only in the depth of the tube, and not on the surface at all. 

 It appears as a finely granular white growth, from which fine filaments 

 extend into the gelatine a short distance from the needle track. These 

 filaments continue to spread and increase in size, and become larger at 

 the outermost ends. They also increase in length as they approach the 

 surface, and give one the idea of a small balsam tree inverted. The 

 central part which was the original needle track has also increased in 

 size and is seen to be composed of fine granules which seem to be sus- 

 pended throughout that portion of the gelatine forming the main 

 stem of the tree, as it were, and also tapering in the depth. After one 

 month's growth the gelatine was seen to be dipping as if drawn in 

 towards the stem portion of the growth. To look down on the growth 

 at this stage it has the appearance of a central, round, finely granular 

 zone, which is well defined, and spreading out from it making a complete 

 circle of rays, are the filaments above described. This dipping gradually 

 progresses until the growth apparently reaches the surface, although it 

 does not spread out thereon, and when that condition is reached liquefac- 

 tion commences and extends down through the heart of the growth or 

 along the old original line of puncture. This is seen to be full of fine 

 granules as above, only some have settled at the bottom of the liquefac- 

 tion, making a slight precipitate. Thirty-six days after inoculation all 



