| Relative Amylolytic Action of Saliva and Diastase of Malt. 127 
The saliva employed in the experiments was filtered, human 
mixed saliva, carefully neutralized and then diluted’; 20 ¢. ¢. saliva 
to 80 c. c. water. As 10. ¢. of this fluid were used in each’experi- 
ment, 1 gram of starch was exposed to the action of 2 ¢."c. of normal 
saliva in a dilution of 1:50. 
The malt extract was prepared from coarsely ground, malted barley 
by extracting 10 grams with 200 ¢. c. water at 40° C. for 3-4 hours ; 
then filtering, neutralizing and diluting to 500 c. ¢., a few drops of 
thymol being added to prevent acid fermentation. The amylolytic 
power of 10 c. c. of this diluted extract was a little more than that of 
a similar quantity of the dilute saliva. Each series of experiments, 
however, was made with different extracts, each of which showed 
considerable variation in amylolytic power. 
A. Amylolytic action at definite temperatures. 
In all of these experiments, the 90 c.c. of fluid containing the 
starch paste was brought to the desired temperature by immersion 
in a large water-bath carefully regulated, the thermometer being 
immersed in the vessel containing the starch paste. In a similar 
manner, the ferment solution was quickly brought to the same tem- 
perature, care being taken in the latter case, that the fluid did not go 
beyond the requisite point. When the temperature became con- 
stant, 10 c. c. of the ferment solution were added and the action con- 
tinued for thirty minutes. 
The results are clearly expressed in the following series of tables: 
Series [.*—Saniva. 
Total amount Starch 
Temperature. Wt. Cu in ¥. reducing bodies. converted. 
10° C. 0°0935 gram. 0°1904 gram. 17°19 per cent. 
20 071227 0°2496 22°46 
40 01410 0°2872 25°83 
50 0°1003 0°2040 18°35 
Series Il.—Sativa. 
Temperature. Wt. Cu in 4. sarncine Lodied: ceneeried: 
20° C. 0°0891 gram. 0°1816 gram, 16°34 per cent. 
30 0°0945 071924 ican 
40 0°1203 0°2448 22°03 
50 0°1419 0°2888 25°99 
55 071129 072296 20°66 
60 0°0451 0°0936 8°42 
65 070125 00282 2°53 
* Tt is of course understood, that the results in any one series are obtained with the 
same ferment solution, 
