on Amylolytic and Proteolytic Action. 137 
Crystallized Total amount Starch 
bile. Wt. Cu in \.* reducing bodies. converted. 
0 per cent. 0°0643 gram. 0°2636 gram. 23°72 per cent. 
0°01 0°0630 0°2584 23°25 
0°02 0°0686 0°2804 25°23 
0:03 0°0693 0°2836 25°52 
0°05 0°0656 0°2688 24°19 
0°10 0:0734 0°3000 27°00 
0°20 0:0665 0°2724 24°51 
0°35 0°0447 071860 16°74 
Here it is plain that a mixture of sodium glycocholate and tauro- 
cholate, in such proportion as they are contained in crystallized ox 
bile, exerts no appreciable retarding influence on amylolytic action 
until present to the extent of 0°35 per cent. On the contrary, 
smaller percentages unmistakably tend to increase the diastatic 
action of the ferment. The solution of crystallized bile had, how- 
ever, a slight acid reaction, and possibly this may have had some in- 
fluence in giving the latter results. The saliva and starch were both 
neutral. 
Experiments were next tried with sodium taurocholate alone, and 
also with sodium glycocholate. Following are the results: 
Sodium 
Total amount 
Starch 
taurocholate. Wt. Cu in \. reducing bodies. converted. 
0 per cent. 0-0787 gram. 0°3212 gram. 28°90 per cent. 
0°3 0°0030 0°0146 1-5) 
0°5 0°0023 0°0112 1:00 
Sodium glycocholate. 
0°5 0-0783 0°3196 28°76 
It is thus plainly evident that sodium taurocholate has a very 
decided action on the amylolytic ferment of saliva, while the same 
percentage of glycocholate is entirely without effect. The retarding 
action of crystallized bile is thus, without a doubt, due wholly 
to the taurocholate. Moreover, even smaller percentages of sodium 
taurocholate retard amylolytic action with almost equal energy. 
The following results were obtained under like conditions as the 
“preceding, except that the 2 per cent. of saliva employed was not 
neutralized. 
Sodium Total amount Starch 
taurocholate. Wt. Cu in ¥%. reducing bodies, converted. 
0 per cent. 0°0590 gram. 0°1212 gram. 21°81 per cent. 
0714 0:0079 0°0192 3°45 
Sodium glycocholate. 
0-20 00758 071548 27°86 
* One-eighth of the entire digestive mixture. 
Trans. Conn. Acap., Vou. VII. 18 Oct., 1885. 
