156 Chittenden and Culbert—Influence of Potassium 
chlorine and removal of the same, before precipitating the urea; 
were carried out with very satisfactory results. Uric acid was 
determined according to the older method of Heintz* with the modi- 
fication of Zablins, and being conducted each time under exactly 
the same conditions and with a urine of approximately the same 
composition, the results are to be considered as strictly comparable. 
Chlorine and bromine were determined in the usual manner with a 
standard solution of silver nitrate; the results, however, are not 
given as they are of value only as essential to the urea determinations. 
Total phosphoric acid was determined by means of a standard solu- 
tion of uranium nitrate.t Phosphoric acid in combination with alkali- 
earths was determined by precipitation with ammonium hydroxide, 
allowing the mixture to stand 24 hours, filtermg the precipitated 
phosphates, washing thoroughly with diluted ammonia, then dissolv- 
ing in a definite amount of dilute acetic acid and titrating with 
uranium solution. Total amount of solid matter contained in the 24 
hours’ urine, was calculated by the use of Christison’s formula. 
The diet specified, was commenced on the 3d day of April; on the 
12th the urine was collected for analysis, the body weight taken and 
the investigation then carried forward without interruption. Table 
No. I. gives the results of the analysis of the urine for nine con- 
secutive days, and shows the average amount of variation to be 
expected under the conditions of the experiment. 
On the 21st of April, 60 grains of potassium bromide were cake 
in divided doses as seen in Table No. Il. The bromide was taken 
about midway between the hours of eating, so that it might not affect 
digestion. On the 22d the dose was increased to 100 grains and then 
to 150, the latter amount being taken daily for three consecutive 
days. Table No. IL. shows the effects of the bromide on the system, 
for the six days it was taken. 
On the first day, the only apparent influence of the bromide is to 
cause a diminution in the amount of phosphoric acid excreted; seen 
both in the total P,O, and in the P,O, in combination with alkali- 
earths. On the second day, the diuretic action of the salt is apparent, 
accompanied with an increase in specific gravity, and a decided 
increase in the amount of urea excreted, together with a slight in- 
crease in the amount of uric acid. Phosphoric acid was still dimin- 
ished in amount. On the third day, the body weight commenced to 
diminish and continued to do so throughout the pe ; diuretic 
* Die Lehre vom Harn, ceo und Leube, p. 94-95. 
+ Die Lehre vom Harn, p, 184. 
