times to the extent of even 1-2 ¢. c. of the sugar solution. In our 
experiments the acid solution was usually yellow or yellowish brown, 
and invariably at the end of the 17 hours contained the flocculent 
precipitate described by Delprat. By nearly neutralizing the solu- 
tion, the amount of this precipitate was considerably increased and 
on then filtering the fluid, after having made it up to a volume of 
200 ¢. c., considerable organic matter was removed. This, we found, 
had a decided influence on the accuracy of the determination, since 
alkaline solutions of this neutralization precipitate appeared to deci- 
dedly retard separation of the cuprous oxide. By paying attention 
to this point, we had no difficulty in obtaining fairly concordant 
results, by the use of the Allihn gravimetric method. 
of Sugar in the Liver, in the presence of Peptones. 187 
e e 9 ‘} 
Experiment 1. 
A large sized rabbit was killed, the blood collected and defibrina- 
ted, the liver quickly removed and finely chopped. Two portions of 
40 grams each were weighed out and treated as follows : 
A. B. 
40 grams liver. 40 grams liver. 
50 c.c. of a 10% solution of peptone. 95 e.c. of water. 
25 e.c. of blood. 
20 c.c. of water. 
These were placed in flasks, and warmed at 40° C. for two hours. 
The liver was in contact with the peptone 40 minutes after the death 
of the animal. A continuous current of air was kept passing through 
A. Following are the analytical results: 
Glycogen; total volume of the resultant sugar solution 200 ¢. c. 
Sugar; total volume of the solution 100 ec. ec. 
Total carbohydrates; volume of the resultant solution 200 e. ec. 
Glycogen A. 
Volume Equivalent Equivalent 
used. Weight Cu. in dextrose. in glycogen. Total amt.* Per cent. 
25 cc. 0°2345 gram. 071209 gram. 0°1088 gram. 0°8704 gram. 5°44 
25 0°2360 071217 071095 0°8760 5°47 
: Glycogen B. 
25 cc. 0°2675 gram. 0°1386 gram. 0°1247 gram. 0°9976 gram. 6:23 
25 0°2659 01377 071239 079912 6-19 
Sugar A, 
25 c. c. 0°2260 gram. O264 gram, 7) ees 0°4656 gram. 2°91 
25 0°2255 OGS "eee ee 0°4652 2°90 
* Total amount of glycogen, dextrose or carbohydrates calculated as dextrose, 
contained in the above volume (100 or 200 ec. c.) and representing, therefore, the 
amount contained in two-fifths of the 40 grams of liver. 
