i. 
of Sugar in the Liver, in the presence of Peptones. 191 
total carbohydrates in those cases where peptone and blood are both 
present. 
A comparison of Seegen’s results* show that while an aqueous solu- 
tion of peptone alone in contact with fresh liver increases somewhat 
the percentage of both sugar and total carbohydrates, the addition 
of blood, kept arterial by the passage of a current of air through 
the fluid, appears to still further increase the percentage of sugar 
and carbohydrates. Seegen, moreover, shows by a blank experi- 
ment, that blood alone in contact with the liver has no more influence 
on the formation of carbohydrates than distilled water. 
Experiment V. 
This experiment was tried mainly to see what influence peptones 
by themselves in the absence of blood, would have on the forma- 
tion of sugar and total carbohydrates. Two portions of sampled 
liver from a large rabbit were treated as follows : 
A. B: 
50 grams liver. 50 grams liver. 
50 c. c. of water containing 2 grams of peptones. 50 c.c. of water. 
The solution of peptone was poured over the liver just 45 minutes 
after the death of the animal. The mixtures were placed in a bath 
at 40° C. for 3 hours, after which they were allowed to stand at the 
temperature of the room for 21 hours. They were then extracted 
and analyzed in the usual manner, with the following results: . 
Glycogen A. 
Volume Equivalent Equivalent Total Per 
used. Weight Cu. in dextrose. in glycogen. amount. cent. 
25 ¢.c. 0°1900 gram. 0:0973 gram. 0°0875 gram. 0°7000 gram. 3°50 
25 071915 0°0980 0°0882 0°7056 3°52 
Glycogen B. 
25 ¢. ¢. 01785 gram. 0°0913 gram. 0°0821 gram. 0°6568 gram. 3°28 
: Sugar A. 
25 ¢. c. 0°4085 gram. Oe Git CRON eg tees 0°8708 gram. 4°35 
10 01735 OSS ya ha ae 0°8870 4-43 
Sugar B. 
10 cc. 0°1745 gram. 0°0892 gram. _-s.: 0°8920 gram. 4°46 
Total carbohydrates A. 
OTC: Cc: 0°1985 gram. OSIO MS cra a= 2°0340 grams. 10°17 
Total carbohydrates B. 
Ove: c. 0°1830 gram. O:0 93) hams ae eee 18740 grams. 9°37 
* Pfliiger’s Archiv, vol, xxv, p.172; ibid, vol. xxviii, p. 125. 
