192 Chittenden and Lambert— Post-mortem Formation 
Average percentage results. 
Amount of 
liver taken Method of treatment. Glycogen. Sugar. carbohydrates. 
50 grams With peptones (A), 3°51 per cent. 4°39 per cent. 10°17 per cent. 
50 Without peptones (B), 3°28 4°46 Fai 
+ 0°23 —0-07 +0°80 
Experiment V1. 
This experiment is practically a repetition of the preceding one, 
excepting that the temperature throughout the experiment was about 
18-20° C. and the length of time 24 hours. This latter point we deem 
of considerable importance, for as the results show, the same increase 
in total carbohydrates in the presence of peptone is apparent here, 
after 24 hours treatment and also in the preceding experiment after 
21 hours treatment, as has been observed at the end of 2 to 3 hours 
in the presence of peptone and blood. For Seegen lays considerable 
stress upon the fact that in many animals the newly-formed carbo- 
hydrates and sugar, supposed to have their origin in the peptones, 
are after, a time decomposed, so that at the end, say of 24 hours, the 
content of sugar and total carbohydrates fall back to their original 
amount, that is, the amount found in the control. Seegen further 
claims that this point speaks strongly in favor of the action of the 
liver, as such, on the conversion of peptone, and rabbits’ liver accord- 
ing to his experiments is no exception to the rule.* The experiment 
# 
was as follows: 
A 
5 B. 
40 grams fresh liver (rabbit). 40 grams fresh liver. 
55 c.c. of water containing 2 grams of peptone. 55 c.e. of water. 
These two mixtures stood at the temperature of the room for 24 
hours, when they were extracted and analyzed with the following 
results : 
Glycogen A. 
Volume Equivalent Equivalent Total Per 
used. Weight Cu. in dextrose. in glycogen. amount. cent. 
25 cc. 0°2767 gram. 0°1436 gram. 0°1292 gram. 10336 grams. 6°46 
Glycogen B. a 
25 c.c. - , 02515 gram. 0°1299 gram. 0°1168 gram. 0°9344 gram. 5°84 — 
Sugar A. 
ZONCAG: 0°2745 gram. 0:14:24 oramspagy 25222 0°5696 gram. 13°56 7 
25 0°2655 O3IS* — 2 ieee 075500 3°43 
Sugar B. 
Ay OME: 0°3210 gram, 01681 gram. Rey es 0°6724 gram. 4:20 
25 0°3260 O-170S ees 0°6836 4°27 — 
Total carbohydrates A. 
MONC. iC: 02100 gram, O1ON9 eran eee ose 2°1580 grams. 13°48 
Total carbohydrates B. , 
10) ese: 0°2013 gram, Om032) cram: = ee==— 2°0640 grams. 12°90 
* Pfliiger’s Archiv, vol. xxv, p. 175. 
+ In this determination, difficulty was experienced in obtaining a good reduction. 
