of Sugar in the Liver, in the presence of Peptones. 197 
three fermentation experiments that we have tried, the sugar solu- 
tion has never contained dextrin. It would of course be more 
natural in seeking for dextrin to look in the glycogen precipitate. 
Finally, it is interesting to notice in connection with this same exper- 
iment, that in B the sum of glycogen calculated as dextrose and the 
sugar after boiling with sulphuric acid equals the total carbohydrates 
found; while in A where peptones are present, the total carbo- 
hydrates more than equal the sum of glycogen and sugar, as is shown 
by the following figures from Experiment V. 
- Sugar not boiled with acid. Sugar boiled with acid. 
Ti oe He fo RE ah es 
Glycogen calculated as dextrose, 3°90 p. ¢. 3°64 p. c. 3°90 p. ¢. 3°64 p. ¢. 
Sugar calculated as dextrose, 4°43 4°46 5°84 5°15 
8°23 8:10 9°74 9°39 
Total carbohydrates found in A 10°17 per cent. 
a3 st ‘ec B 9°37 
This shows an apparent increase in total carbohydrates of 0°43 
per cent. (in A) under the influence of the peptones; this being the 
difference between the total carbohydrates found, and the sum of 
glycogen calculated as dextrose and the sugar after boiling with 
dilute acid. In B on the other hand, the total carbohydrates found 
and the sum of glycogen and sugar differ but 0°02 per cent. 
Experiment VIII. 
This experiment was tried mainly to verify the preceding state- 
ments regarding the liver sugar. 
40 grams of liver from arabbit were finely divided and placed in a 
flask with 50 ¢.c. of water. The mixture was then warmed at 38- 
40° C, for 2 hours, when it was boiled and extracted in the usual 
manner. The liver was placed in the warm bath just 25 minutes 
after the death of the rabbit, consequently 2} hours intervened be- 
tween the death of the animal and the stopping of ferment action. 
Following are the results of the analysis: 
Glycogen. 
Volume A Equivalent Equivalent Total Per 
used, Weight Cu. in dextrose. in glycogen. amount. cent. 
25 ec. 0°2990 gram. 01560 gram. 01404 gram. 11232 grams. 7:02 
Sugar. 
HOC. Cc. 00915 gram, 070466 gram. 22222 04660 gram. 2°91 
Sugar after boiling with dilute H.SO4. 
19 c.e. 0°1145 gram. Oi0o S82) ora eee = 0°5820 gram. 3°63 
Total carbohydrates. 
HOY; .C: 0°1790 gram, Qe0 NIG s oT ay te ae sere 1°8520 grams, 11°45 
