200 Chittenden and Lambert— Post-mortem Formation 
empty and the small intestines likewise. The sampled liver was in 
the bath at 40° C., 25 minutes after the death of the animal. 
A. B. 
40 grams of liver. 40 grams of liver. 
25 grams of blood. 135 c. ec. of water. 
100 c. ec. of a 2 per cent. solution of peptone. 
10 c. c. of water. 
These mixtures were warmed at 40° C. for 2 hours, with a current 
of air passing through A, after which they were extracted and treated 
in the usual manner. Glycogen was not found in sufficient quantity 
for determination in either A or B. An accident happened to the 
sugar solutions, so that the absolute amount of sugar could‘not be 
estimated, but the relative reducing power of the sugar solution, 
before and after boiling with dilute sulphuric acid, was accurately 
determined. 
Total carbohydrates A. 
Volume Equivalent Total 
used. Weight of Cu. in dextrose. amount. Per cent. 
25 ¢. ¢c. 0°1070 gram. 0°0545 gram. 0°4360 gram. 2°72 
Total carbohydrates B. 
25 ©. ¢. 0:0865 gram. 0:0441 gram. 0°3528 gram. 2°20 
Sugar A, 
25 ¢. ¢c. 0°1045 gram. 0°0529 gram. —- Before boiling with dilute acid. 
25 0°1479 00753 After boiling with acid. 
Sugar B. 
25 ¢. ¢. 0°1453 gram. 0:0740 gram. Before boiling with dilute acid. 
25 071925 0°0986 After boiling with acid. 
In this case, the total carbohydrates show an increase of 0°52 per 
cent. in the presence of peptone. The sugars, unlike the result in 
the preceding experiment, show a very great increase in reducing 
power, on being boiled with dilute sulphuric acid. In 4, the rel- 
ative reducing power before and after boiling with the acid, is 
70-6 :100 and in B 75:4:100. Hence in this experiment it would 
appear that the lower reducing body, presumably maltose, is present 
in great excess. 
| Experiment XI. 
In this experiment, a liver was taken from a freshly killed lamb, 
sampled in the usual manner and then warmed at 40° C., with and 
without peptone, for 4 hours. The liver was in the bath and mixed 
with the peptone solution just one hour and 20 minutes after the 
death of the animal. In this experiment no blood was used. 
—_ 
eS 
