202 Chittenden and Lambert— Post-mortem Formation 
Amount of , ota 
liver taken. Method of treatment. Glycogen. Sagar. carbohyarates. 
50 grams. With peptones (A). 0 1°10 per cent. 1°53 per cent. 
50 Without peptones (B), 0 0°84 0:96 
+ 0°26 +0°57 
Increase in both sugar and total carbohydrates is to be noticed 
in A. The increase in total carbohydrates, moreover, is twice as 
great as the increase in sugar. 
Experiment XU. 
With a calf’s liver, from a freshly killed animal, obtained at the 
slaughter house. 
C. 
50 ae of liver. 50 ie of liver. 50 grams of liver. 
100 c.c. of water. 100 c¢.c. of water. 100c.c. of a 2 per cent. solution of peptone. 
A was warmed at 40° ©. for 1 hour and 20 minutes, after which it 
was extracted and analyzed; Band C on the other hand, were heated 
for 1 hour and 30 minutes at the same temperature, after which they 
were allowed to stand for 16 hours at about 18-20° C. before being 
extracted. The special object in view was to ascertain the influence 
of time on the disappearance of glycogen. 
Following are the results obtained : 
Glycogen A. 
Volume Equivalent Equivalent Total Per 
used. Weight of Cu. in dextrose. in glycogen. amount. ‘ «cent. 
25 ¢. ¢. 0:0230 gram. 0°0125 gram. 0°0112 gram. 0°0896 gram. 0-44 
Glycogen B. 
25 @. Cc. 0:0105 gram. 0°0063 gram. 0:0056 gram. 0°0448 gram. 0°22 
Glycogen C. 
20 €.1G; a mere trace only. 
Sugar A. 
25 ©. C: 071900 gram. 00973 gram. eae 0°3892 gram. 1:94 
After boiling with dilute H2SO,. 
25 ¢. C. 0-2100 gram. OslON Sera eee 04316 gram. 2°15 
Sugar B. 
25 ¢. ¢. 0°2445 gram. Oal2620e rams eee 0°5048 gram. 2°52 
After boiling with dilute H.SO,. 
25 ¢. ¢. 0°2535 gram. (PUGH) reangehins | = S558 0°5240 gram. 2°62 
Sugar C. 
25 ©. C. 0°2315 gram. 071198 gram. cyons 0°4792 gram. 2°39 
After boiling with dilute H2SOx. 
25 ¢.¢. 0°2620 gram. O:Sooramnte Veer 0°5428 gram. 2°71 
Total carbohydrates A. 
25 ¢. ¢. 0°1210 gram. 0:0616 cramer Seyeee=— 0°4928 gram. 2°46 
Total carbohydrates B. 
20) ChG. 0°1410 gram. O07 18 orame opeeeeee= 05744 gram. 2°87 
Total carbohydrates C. 
25 cc. 01405 gram. OOo pram eee 0°5720 gram. 2°86 
