of Sugar in the Liver, in the presence of Peptones. 203 
A, B. Cc. 
Without peptones, Without peptones, With peptones, 
1 hour 20 min. 14g hours at 40°C. 16 hours at 40° C. 
at 40° C. and16h.at20°C. and 16h. at 20°C. 
(CIN@S2G0 5 as See eee 0-44 per cent. 0°22 per cent. 0 
Sugar before boiling with H.SO, 1:94 2°52 2°39 per cent. 
Sugar after boiling with H,SO4- 2°15 2°62 2°71 
Qotal carbohydrates_-__--.--.--- 2°46 2°87 2°86 
A comparison of these results shows no increase whatever in total 
carbohydrates in the presence of peptone (6 and CU.) Somewhat 
strange is the apparent increase of 0°41 per cent., in the absence of 
peptone after standing 24 hours. The percentage of glycogen is 
diminished one-half after standing 24 hours, and, as has been noticed 
several times before, it is’ still further diminished in the presence of 
peptone. Consistent with the theory of the formation of liver sugar 
from the hepatic glycogen, is the fact that in this experiment increase 
in sugar accompanies decrease in glycogen. This is shown best in 
the percentages of sugar, after boiling with dilute sulphuric acid. 
The relative reducing power of the sugar solutions, before and after 
boiling with dilute acid, is in A 90°1: 100, in B 96-4: 100, and in C 
88°3: 100. In other words the liver sugar in B after standing 24 
hours is composed mainly of dextrose, the lower reducing body 
present in larger quantity in A having been gradually changed by 
longer contact with the liver tissue. In C, however, although the 
time was the same as in B, the presence of peptone, while it appears 
to increase the decomposition of glycogen and thus the actual amount 
of sugar formed, tends to prevent apparently the conversion of the 
lower reducing sugar into dextrose; hence in (' the percentage of 
sugar before boiling with acid is less than in B, while after treat- 
ment with acid it is greater. 
Following is a resumé of the various results obtained with peptones. 
Experiment 1.—Rabbit’s liver. 
Liver Total 
taken. Treatment. Glycogen. Sugar. carbohydrates. 
40 grams. With peptones and blood. 5°46 Z 2°91 % 11°08 4 
40 Without ee ae 6°21 2°74 10°75 
2 hours at 40° C. —0°75 +0:17 +0°33 
Experiment .—Rabbit’s liver. 
mayer ; Votal 
taken. Treatment. Glycogen. Sugar. carbohydrates 
40 grams. With peptones and blood. 1:46 Z 3°26 % 14:15 ¢ 
40 Without oe be 8:09 2°75 13°55 
2 hours at 40° C, —0°63 +0°51 + 0:60 
