‘ 
910 Kiihne and Chittenden— Globulin and Globulose Bodies. 
Digestion of Globulin. 
The preparation proved to be extremely difficult of digestion; this 
fact, however, enabled us to remove from the globulin the magnesium 
sulphate still remaining, since the large quantity of dilute hydro- 
chloric acid added with the small amount of pepsin, failed at first to 
dissolve any of the albuminous matter. The undigested residue was 
then finally treated with an artificial gastric juice much richer in 
pepsin. 250 grams of the powdered globulin were at first warmed 
with 5 litres of 0°2 per cent. hydrochloric acid for 24 hours, whereby 
the substance swelled up to double its former bulk, but only a trace 
was dissolved; barely enough for a filtered portion to give with 
nitric acid and heat a slight turbidity. By the addition of 200 ¢. ¢. 
of normal gastric juice* to the swollen mass and warming it at 
40° CO. for 24 hours longer, the albuminous matter was not appreci- 
ably changed; a filtered portion, however, became turbid on neu- 
tralization and a small amount of albumose could be detected both 
with sodium chloride and with nitric acid. The entire mass of swollen 
globulin was then collected on a cloth filter, washed thoroughly with 
0-2 per cent. hydrochloric acid and warmed again at 40° C, for six 
days with 4 litres of a particularly active gastric juice, contain- 
ing 0°4 per cent. hydrochloric acid and 0°45 per cent. of solid matter. 
By this last treatment, a large amount of globulin was dissolved. 
As the digestive mixture would not filter through cloth it had to be 
neutralized directly with sodium hydroxide, which gave an abundant 
neutralization precipitate of so-called parapeptone, easily collected, 
and from which the fluid filtered perfectly clear. In order to obtain 
more material for study, the neutralization precipitate, together with 
the unaltered globulin, was treated a second time with 3 litres of the 
same active gastric juice for several days, by which the amount of 
globulin was reduced more than half, as shown by repeated neutrali- 
zation. 
The two neutralized, digestive fluids obtained in this manner, were 
alike in all respects; noticeably so in the remarkable fact that when 
weakly alkaline, they became turbid at 53° C., which turbidity 
increased as the solutions were heated to boiling. Furthermore, 
when made faintly acid, heat produced in both solutions an abundant 
flocculent precipitate which had the properties of coagulated albu- 
min. This coagulun from digested globulin was purified by succes- 
sive washings with boiling water, alcohol and ether. The following” 
table shows the composition of the substance. 
* See Zeitschrift fiir Biologie, Band xix, p. 184. 
