212 Kiihne and Chittenden— Globulin and Globulose Bodies. 
Globulose Bodies. 
After removal of the above mentioned coagulum, the solution re- 
mained perfectly clear at all temperatures up to 100° C., even when 
rendered more strongly acid and also on subsequent neutralization. 
On the addition of nitric acid, however, to the cold solution, a pre- 
cipitate was formed which disappeared on the application of heat, 
reappearing as the mixture became cool. 
Crystals of salt alone, produced a heavy precipitate in the solution, 
while salt and acetic acid gave a still further precipitate, and when 
these reagents failed to cause any further precipitation, nitrie acid or 
metaphosphoric acid would still give a noticeable turbidity. 
In order to separate the globulose bodies from one another, the 
entire solution was concentrated on a water bath to the consistency 
of a thin syrup and then rubbed up in a mortar with salt in sub- 
stance (the fluid being perfectly neutral), complete saturation being 
insured by long standing with an excess of salt crystals. The pre- 
cipitate so formed being separated by filtration, the filtrate was par- 
tially precipitated by the cautious addition of 30 per cent. acetic acid 
saturated with salt, whereby a mixture of proto- and deutero- 
globulose was separated. After removal of this precipitate the 
filtrate was finally treated with more of the above acetic acid until 
nothing further was precipitated. 
The various precipitates were then subjected to strong pressure to 
remove as much of the salt-saturated fluid as possible, then dissolved 
in water and dialyzed for the complete removal of the salt and to 
separate heteroglobulose. 
Protoglobulose. 
“This body, precipitable by sodium chloride alone, was purified by 
saturating the first dialyzed solution again with salt, then dialyzing 
a second time under repeated changes of reaction, by the alternate 
addition of acetic acid and sodium carbonate and finally by neutral 
reaction, until all chlorine was removed from the solution and the 
admixed heteroglobulose completely separated. The clear filtered 
fluid was then concentrated, after which the protoglobulose was pre- 
cipitated with alcohol, washed with alcohol and ether and so ob- 
tained as an almost white powder. The substance, so prepared, gave 
when rubbed up with cold water, a filtrate not quite clear and witha 
noticeably alkaline reaction. It differed from a solution of proto- 
albumose from fibrin in one respect, viz: that on boiling in the pres- 
ence of a small amount of sodium chloride it became quite turbid, 
