Kiihne and Chittenden— Globulin and Globulose Bodies. 215 
simply by concentration of the solution freed from chlorine, pre- 
cipitation with alcohol and washing with ether. The ash (1°17 per 
cent.) consisted only of calcium phosphate and a trace of sulphate. 
Leteroglobulose. 
This body was obtained from the gummy precipitate which sepa- 
rated, during dialysis, from the solution of the first precipitate 
thrown down from the neutralized digestive fluid by salt alone. 
The sticky mass was separated from the sides of the parchment 
tubes, dissolved in sodium chloride of from 3 to 5 per cent., repre- 
cipitated by saturation of the solution with salt, the precipitate again 
dissolved in dilute salt solution and the substance finally separated 
by long continued dialysis in running water. After thorough wash- 
ing with water, alcohol and ether it appeared as a light, white 
powder, not unlike heteroalbumouse in general behavior and _ reac- 
tions. After each precipitation and treatment with dilute sodium 
chloride, heteroglobulose left a residue, which like dysalbumose was 
readily soluble only in dilute acids. From the following analysis 
‘it is to be seen that the preparation, in spite of its long continued 
and repeated dialysis, contained 2°03 per cent. of ash, which con- 
sisted mainly of calcium carbonate with a small amount of phos- 
phate and sulphate. 
The composition of the three globulose bodies shows the same 
slight differences as noticed in the case of the various albumose 
bodies (from fibrin). Unlike the latter, however, the content of car- 
bon in the globulose bodies never falls below 51 per cent., and fur- 
thermore it is always higher than that of the globulin from which the 
globulose was derived. The percentage of nitrogen, which in the 
albumose bodies was found a little higher than in fibrin, exceeds that 
of the globulin more yet, in some cases by more than 1 per cent., 
and the same holds true of the percentage of sulphur. In contrast 
to the albumose bodies, the percentage composition of the globulose 
bodies gives no grounds whatever for the assumption that they arise 
from the digested globulin by simple hydration. It must not be for- 
gotten, however, that the digestion of globulin by gastric juice is a 
process quite different from that of fibrin digestion and one hitherto 
much less clearly understood, since besides the globulose bodies 
there is formed a large quantity of a substance which is separated by 
boiling and which resembles ordinary coagulated albumin. Some- 
thing similar, indeed, has been known ever since Briicke’s study of 
fibrin digestion, but it has long been accepted that the coagulum 
