218 Kiihne and Chittenden— Globulin and Globulose Bodies. 
obtained by Briicke on boiling the neutralized digestive fluid, arose 
from the globulin present in the fibrin employed, which had not been 
previously washed with salt water. Globulin, moreover, yields this 
body in much greater quantity, even after several days’ exposure to 
the action of an energetic gastric juice and it was still found abund- 
antly among the products of a second digestion of the first neutral- 
ization precipitate. 
Comparison of the Analyses. 
a | oe = 
2 : gte z | 
g Pins g 48 g eee Ee, 
a loess. | ogg || ee oe ee )a58. gem | & 
=| Salt SS o s95e 25 B 
S Soo Roce \uaee ri | B8e2 | ge8°| 2 
o SES "bp Qe eo) | Bars | Wad a 
C | 51:14 | 52°03 | 51°57 | 51°52 | 52-10 © | 52°13 | 50°88 | 52°68 
H 700 | 6:93 6:98 6:95 6:98 oH | 683) 689| 683 
N 14:64 | 15°89 | 16°09 | 15:94 | 16:08 N | 1655 | 1708 | 1691 
Ss 1°67 1-80 2-20 1-86 2°16 S | (1099) 1-23 1:10 
O | 25°55 | 23°35 |! 23-16 | 23-73 | 22°68 O | 23:40 | 23°92 | 29-48 
In this review of the composition of globulin and of the products 
of its digestion we have included also an analysis of fibrin, of a 
fibrin-albumose and of hemialbumose from the urine of a person 
with osteomalachia, 
We call attention again to the latter because its surprising corre- 
spondence, especially to heteroglobulose, appears to confirm the be- 
lief, expressed in our former paper that the difference in the albumose 
from urine and that from fibrin depends on the formation of the 
former from an albuminous body, whose digestion, at least as regards 
the formation of albumose bodies, was then unknown, and for which 
we had already turned to globulin. 
In order to gain further information concerning the cleavage of 
globulin in the process of digestion, the remaining material was used 
in the following experiments. 
1. Heteroglobulose dissolved in 0°3 per cent. sodium carbonate 
and warmed at 40° C. for fourteen days with pure trypsin (with the 
addition of thymol, as usual) remained perfectly clear, even after 
neutralization, and failed to yield afterwards any body resembling 
antialbumid. Among the products of the digestion, there was found 
in addition to an abundance of antipeptone, only a trace of leucin, no 
tyrosin whatever, while with bromine water the alcoholic extract, 
* Compare Zeitschrift fiir Biologie, Band xix, p. 202. + Ibid., Band xx, p. 40. 
’ $+ According to Hammarsten. 
