Kiihne and Chittenden— Globulin and Globulose Bodies. 219 
which had been dissolved in water after driving off the alcohol, 
became simply a little darker, but not rose-colored or violet. Hence 
heteroglobulose is to be considered as belonging to the anti group. 
2. Protoglobulose, which still contained some heteroglobulose, 
‘when treated in the same manner with trypsin, behaved similarly 
but afforded besides an abundance of leucin, also some tyrosin and 
an extract which became deep violet on the addition of bromine 
water. Hence protoglobulose gives evidence of belonging to the 
hemi group. 
Finally, we submitted to the digestive action of trypsin the third 
and fifth precipitates (so-called parapeptone) which were separated 
in continually decreasing quantities by neutralization, after renewed, 
energetic pepsin digestion of the original globulin. Both failed to 
yield any coagulum during their digestion with 0°3 per cent. sodium 
carbonate, and after the trypsin had acted for fourteen days, neu- 
tralization with acetic acid yielded a heavy precipitate, while consid- 
erable antipeptone was found in the solution. Although the diges- 
tion of the third neutralization precipitate still afforded a trace of 
leucin and tyrosin without giving any reaction with bromine, no 
leucin, tyrosin or a substance colored by bromine water could be 
obtained from the precipitate separated after the fifth pepsin diges- 
tion. 
Hence globulin, like fibrin and other albuminous bodies, yields dur- 
ing pepsin digestion at the last only bodies of the anti group, which 
are peptonized, though slowly, by trypsin, but yield no further cleav- 
age products. 
