228 Kiihne and Chittenden—Peptones. 
The peptone thus obtained appears (when dried at 105° C.) asa 
dry, light yellowish powder. It can be preserved in this form only 
when most tightly stoppered. In the air it soon forms large balls, 
becomes sticky like pitch and melts to a tough mass which does not 
become visibly thinner. What is truly surprising is the behavior of 
the peptone towards water. <A bit of the powder wet with a small 
drop of water hisses and steams like phosphoric anhydride when 
moistened, and when this, or the powdered but not absolutely dry prepa- 
ration which no longer hisses, is dissolved in water, a development of 
heat is to be noticed. We have observed the same remarkable pecu- 
liarity in anti-peptone to be described later. 
Analysis of the preparation (Amphopeptone B) dried at 105° C. 
in vacuo, gave the results tabulated in the accompanying table. 
Amphopeptone (6). 
After these results were obtained, an attempt was made to reduce 
the ash content of the preparation by repeated precipitation with 
alcohol, which succeeded so well that the substance when dried iz 
vacuo over sulphuric acid, later at 106° C. until of constant weight, 
contained then 2°15 per cent. of ash instead of 3°25 per cent. 
I. 0°5500 gram of this preparation gave 0°3400 gram H,O = 6°86 
per cent. H and 0:9566 gram CO, = 47°43 per cent. C, 
II. 0°7121 gram of substance gave 98:4¢.c. N at 16°4° C. and 
765°5mm. pressure = 16°49 per cent. N. 
III. 0°7839 gram substance gave 0°0169 gram ash = 2°15 per cent. of 
ash, 
Therefore in the ash-free substance (Amphopeptone b) there are— 
48-47% O, 7.02% H, 16°838% N. 
Il. Antipeptone. 
As with amphopeptone, we have formed in various digestion exper- 
iments several preparations of antipeptone by the action of trypsin. 
These preparations have been studied both after purification with — 
alcohol and after further purification with phosphotungstic acid, but — 
in every case after complete removal of the albumose bodies. We 
have not attempted to meet the objection that the antipeptone 
is not formed exclusively from the digested fibrin, but in part trom 
the albumins of the pancreas. This would have necessitated experi- 
ments with pure trypsin, which seemed too costly to undertake. In- 
stead of this we have studied a peptone which we shall distinguish 
