KR. H. Chittenden—Dehydration of Glucose. 255 
acid. Somewhat in accord with Pavy’s results, however, is the fact 
that while 200 milligrams of glucose were introduced into the stom- 
ach mixture and 150 milligrams with the intestines, only 152°4 milli- 
grams were recovered from the former and 106 milligrams from the 
latter, although the residues after heating with sodium sulphate, were 
repeatedly and thoroughly washed with hot water. Assuming that 
this loss of sugar in the two cases is really due to change of glucose 
into lower reducing bodies, the relative reducing power of the sugar 
before and after contact with the stomach and intestines would be 
100: 76°2 and 100: 70°6 respectively. But if there had been any 
such change in reducing power, the treatment with sulphuric acid 
would certainly have indicated it. 
Second experiment.—A rabbit in full digestion was killed and half 
of the stomach and a portion of the small intestine were cleaned and 
finely divided. The stomach tissue was then heated at 48°8° C. for 
one and one-half hours, with 70 ¢. c. of water containing 0°200 gram 
of glucose and the portion of intestine for the same length of time, 
with a like amount of glucose. Treated then in the same manner as 
the preceding solutions, the following results were obtained : 
STOMACH. 
a. Before treatment with sulphuric acid. 
25 c. c. gave 0:0901 gram Cu =0:0460 gram dextrose x 4=0°1840 gram dextrose. 
b. After treatment with sulphuric acid.» 
25 c. c. gave 0°0882 gram Cu =0°0450 gram dextrose x 4=0°1800 gram dextrose. 
INTESTINE. 
a. Before treatment with sulphuric acid. 
25 c.e. gave 0°0690 gram Cu =0°0353 gram dextrose x 4=0°1412 gram dextrose. 
b. After treatment with sulphuric acid. 
25 ¢.c. gave 0°0726 gram Cu =0°0371 gram dextrose x 4=0'1484 gram dextrose. 
In this experiment, a larger amount of sugar was recovered in the 
case of the stomach tissue than in the preceding experiment, but in 
neither the stomach or intestine is there any evidence of change in 
the reducing power of the sugar before and after treatment with sul- 
phuric acid. In this conneetion it is to be remembered, that the only — 
ground for belief in the existence of a dehydrating ferment in the 
stomach is the change, noticed by Pavy, in the reducing power of 
the sugar under the above method of treatment. Our method of 
treatment with sulphuric acid, moreover, both as to the length of 
time the mixtures were heated and the strength of acid employed, was 
in accord with the method used by Pavy. In addition, the same 
