Antimonious Oxide on Metabolism. 295 
‘due heated carefully until the organic matter was completely oxidized. 
The fused mass was then dissolved in water and diluted to 250 c.c. 
Of this, 100 ¢.c., representing 20 c.c. of the original urine, were used 
for the sulphur, while the second 100 c.c. were used for the 
phosphorous, determination. For sulphur, the 100 cc. were acidi- 
fied with hydrochloric acid and evaporated to dryness on a water 
bath in order to remove all nitrate and nitrite. The residue was then 
dissolved in water acidified with hydrochloric acid, and the sulphuric 
acid precipitated with barium chloride in the usual manner. For 
phosphorus, the 100 c.c. were acidified with nitric acid, evaporated 
to dryness, the residue dissolved in water, acidified with nitric acid 
and the phosphoric acid precipitated with molybdenum solution. 
This precipitate was then dissolved in a dilute solution of ammonia, 
the phosphoric acid reprecipitated as ammonio-magnesium phosphate, 
and the phosphorus finally weighed as magnesium pyrophosphate. 
Chlorine was determined volumetrically in the usual manner, with 
a standard solution of silver nitrate, after destruction of the organic 
matter by fusion with potassium nitrate, etc. 
The results, expressed in grams per 24 hours, are shown in the ac- 
companying tables. The 24 hours’ urine represents the quantity passed 
from 9 A. M. of one day to 9 a. M. of the next. As, however, the ani- 
mal was not always regular in its passage of urine, it frequently 
happened that the quantity on one day would be very small, while on 
the next it would be correspondingly increased, without any change 
in specific gravity, and with a daily average corresponding to the 
normal, as for example on May 25th and 26th. 
In order, therefore, to obviate the difficulty which this irregularity 
tends to introduce into the results, we have added to the table a daily 
average of each three days results; a study of which shows plainly 
that antimonious oxide, in the present experiment at least, does 
not have any noticeable influence on the excretion of any of the ele- 
ments determined. Numerically, there is a slight increase in the 
amount of nitrogen excreted during the taking of the antimony, but 
the increase is noticeable only in the grand average and is altogether 
too small to be of much significance. Further, it is to be noticed that 
the average for the two series does not show any corresponding in- 
crease in sulphur. If antimony causes an increased excretion of 
nitrogen, it means an increase in proteid metabolism, which should in 
turn give rise to an increased excretion of sulphur and phosphorus. 
It is to be noticed in the daily results, that the excretion of sulphur 
nd phosphorus runs parallel with the excretion of nitrogen; an in- 
