Compounds of Albumin and Myosin. 319 
solution purified simply by three days’ dialysis, Loew found that a 
1 per cent. solution of silver nitrate gave no precipitate whatever 
in a 5 per cent. solution of albumin. On adding a little dilute 
sulphuric acid to the albumin solution, however, and then pouring 
the mixture into the silver solution a precipitate was obtained, 
which on thorough washing and drying was found to contain 2°17 
per cent. of Ag; while a second preparation made by using a little 
less sulphuric acid contained 2°40 per cent. of Ag. By precipitat- 
ing the albumin solution directly with a 5 per cent. solution of silver 
nitrate, without the addition of any acid, the albuminate was found 
to contain in one case 4°39 per cent. Ag, in a second case 3°91 
per cent. Ag. Dissolving the freshly precipitated albuminate formed 
in this manner, in dilute ammonia and then reprecipitating it by the 
addition of dilute sulphuric acid to slight acid reaction, the albumin- 
ate was found to contain 4°64 per cent. of Ag. 
Loew sees in these results a confirmation of Harnack’s views as to 
the copper albuminates, and an assurance that the molecular weight of 
albumin corresponds to Lieberkiihn’s formula three times enlarged. 
Using an albumin solution purified as in our previous experiments 
and adding to it a 10 per cent. solution of silver nitrate as loug as a 
precipitate was formed, four distinct series of albuminates were made* 
representing four distinct preparations of egg-albumin. These were 
all washed free from silver and also from any adhering albumin, dried 
at 110° C. until of constant weight and the silver determined by 
simple ignition. 
Series I. 
No. Amt. Sub. taken. Wt. Ag. Per cent. Ag. 
la 0:5900 gram. 0:0242 gram. 4:10 
b 05625 00230 4:08 
2a -0°5760 00230 4-11 
b 0°7548 00305 4:04 
3a 09005 0-0362 4-02 
b 0:°7973 00325 4:07 
Seriss IT, 
la 05859 0:0245 4:18 
b 06967 0-0290 4°16 
2a 0°9473 00385 4-06 
b 06621 00270 4-07 
3a 0°6455 0°0266 . 4°12 
b 0:7000 — 00285 4:07 
* The silver compounds were all made and analyzed by Mr. T. S. Bronson of this 
laboratory. 
