Compounds of Albumin and Myosin. 325 
With Cu(C,H,9,),. 
No. Amt. Sub. taken. Wt. CuO. Per cent. CuO. Per cent. Cu. 
Ta 0:7360 gram. 0:0161 gram. 2°18 1:73 
b 0:7088 0:0154. 2°17 £73 
8a 0°7789 0°0174 2°24 STEKS 
b 0°8111 0:0181 2°23 7p 
9a 0:7376 t 0:0169 2°29 1°81 
b 0°6155 0:0188 2°24 1:78 
10a 0°-7815 0°0191 2°44 1:94 
b 0-874 0:0213 2°43 1:93 
lla 0:8048 0:0170 2-11 1°67 
b 0°8308 0:0174 2:10 1°67 
12a 0:6780 0:0150 2°21 Lio 
b 0°7695 0:0168 2:18 1°74 
Comparing these results with one another, there is to be seen a 
very noticeable lack of agreement in composition, and further it is 
to be seen that. the myosin-copper compound has on an average a 
somewhat higher content of copper than the albumin-copper precipi- 
tate. The average composition of all the copper myosins shows about 
1-42 per cent. of Cu, and deducting 0°25 per cent. of ash, the average 
content of Cu would be 1°17 per cent. Examining the individual 
results, it is apparent that the compounds made from the same myosin 
solution are approximately, at least, the same in composition and 
without doubt the difference in the composition of compounds made 
from different myosin solutions is due to variation in the concentra- 
tion of, and possibly also in the reaction of, the myosin-containing 
fluid. It would appear as if variations in the conditions of precipita- 
tion made a greater difference in the case of the myosin-copper com- 
pounds than in the compounds of copper with albumin. Several 
times, also, we have found that our myosin-copper precipitate con- 
tained sulphuric acid, even after thorough washing and when the 
wash-water was proved to be entirely free from any reaction with 
barium chloride. 
One such compound, after drying at 110° C., was analyzed with 
the following results: 
0°7240 gram substance* gave 0:0343 gram BaSO, = 1:63 per cent. SOs. 
0°8420 gam substance gave 0:0331 gram BaSO, = 1°73 per cent. SOs. 
0°4705 gram substance} gave 00084 gram CuO = 1°78 per cent. CuO. 
0°6011 gram substance gave 0:0107 gram CuO —=—- =_: 1°78 per cent. CuO. 
* Roasted and then ignited with pure sodium carbonate, the residue dissolved in 
hot water acidified with hydrochloric acid and precipitated with barium chloride. 
+ Ignited, the residue dissolved in nitric acid, precipitated with hydrogen sulphide, 
etc. 
