XXI.—Ecc-ALBumiIn anp A.tsumosEs. By R. H. CuirrenpEeNn 
AND Prrcy R. Bouron, Pu.B. 
Ever since the albumose bodies were first separated from the pro- 
ducts of fibrin digestion* with pepsin-hydrochloric acid, it has been 
our intention, already expressed, to subject the various individual 
albuminst to the action of purified pepsin under like conditions, and 
thus ultimately to acquire a comparative knowledge of the albumose 
bodies obtainable from these different sources. Already the albumose 
bodies from fibrin and the globuloses{ have been subjected to a care- 
ful study and we present here the result of a study of the albumoses 
from egg-albuniin. 
In doing this, we have to report at the same time, the results of a 
study of the composition of egg-albumin itself. For we have made 
it a rule, in the series of experiments shortly to be described, to 
analyze a sample of each lot ofalbumin prepared for digestion, In this 
manner we have obtained data for a direct comparison of composition 
between the original sample of albumin and the products formed by 
its digestion. This we have deemed of considerable importance, for 
the data so obtained may throw considerable light on the nature of 
the changes involved in the formation of the albumoses; particu- 
larly, as to whether they are hydrolytic in their nature. 
Four distinct samples of albumin were prepared, three of which 
were prepared in large quantities and served as material for the subse- 
quent digestions. 
Albumin A. 
This was a preparation of coagulated egg-albumin, prepared espec- 
ially with the view of obtaining a product wholly free from globulin. 
The method employed was essentially that recommended by Ham- 
marsten.§ The whites of 120 eggs were freed from the yolks, then 
*W. Kiihne and R. H. Chittenden, Ueber Albumosen, Zeitschrift fiir Biologie, Band 
26.6), 5 1S 
+ W. Kihne and R. H. Chittenden, Ueber die nachsten Spaltungsproducte der 
Hiweisskérper, Zeitschrift fir Biologie, Band xix, p. 159. 
{ W. Kiihne and R. H. Chittenden, Globulin und Globulosen, Zeitschrift fir Biologie, 
Band xxii, p. 409. 
§ See K. V. Starke, Beitrage zur Kenntniss des Serum- und Kialbumins, Jahres- 
bericht fiir Thierchemie, 1881, p. 18. 
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