336 Chittenden and Bolton—Egg-Albumin and Albumoses. 
determined by the magnesium sulphate method, never reaches 1 per 
cent., but averages only 0°667 per cent., it seems probable that the 
greater portion is separated by the acetic acid. Further, Dill!ner has 
found that on the dialysis of a neutralized egg-albumin solution, the 
matter which separates out after a few days dialysis, is only in part 
globulin, but consists, in addition, of a somewhat insoluble body rich 
in sulphur. Hence, the substance which separated in our dialyzers, 
after precipitation with acetic acid and neutralization, may not have 
been composed wholly of globulin. The following table shows the 
composition of the uncoagulated albumin B. 
Albumin C. 
This preparation was much the same as albumin B, except that it 
was finally coagulated. Globulin was separated by acetic acid, the fil- 
trate neutralized, again filtered and the fluid dialyzed in running water 
until all soluble salts were removed. The albumin was then coagu- 
lated by being poured into a large volume of boiling water acidified 
with acetic acid. A sample, after drying at 106° C. im vacuo, was 
found to have the composition shown in the accompanying table. 
Albumin D. 
This sample of albumin was prepared in exactly the same manner 
as albumin A; the globulin removed by magnesium sulphate, the 
albumin precipitated by sodium sulphate and after dialysis, coagu- 
lated as already described. Its composition is shown in the follow- 
ing table. 
Comparing now, the results of the analysis of these four samples of 
albumin, it is seen that the first three agree almost exactly in com- 
position, while the fourth shows a somewhat lower content of carbon. 
A B C D Average. 
C 52°21 52°33 52°46 51°74 52°18 
H 6°96 6°98 7:00 6°81 6°93 
N 15°80 15°89 15°88 15°68 15°81 
s 1°94 1°85 1°69 2:02 1°87 
O 23°09 22°97 22°97 23°75 23°21 
Ash 0°37 | O17 0°45 
Further, the coagulated products (A and C) do not differ at all in 
composition from the non-coagulated albumin B. 
Schiitzenberger,* as a result of his work on proteid matter, ascribed 
* Bulletin de la Société Chimique de Paris, T. 23 et 24, 
