Chittenden and Bolton—Kgg-Albumin and Albumoses. 339 
to albumin the formula C,,,H,,,N,,0,,5,, which requires a content of 
carbon not far different from the average of our results, but which 
on the other hand demands a content of nitrogen nearly 1 per cent. 
higher than we found. The well-known Lieberkiiln’s formula requires 
53°59 per cent. of carbon, or 1 per cent. more than was found in our 
highest result. Harnack’s formula for albumin,* C,,,H,,,N.,0,,5,, with 
a molecular weight of 4618, based on a study of the copper compounds 
of albumin, requires too high a content of carbon and altogether too 
low a percentage of sulphur. Lieberkiihn’s formula requires 1°98 per 
cent. of sulphur, while Harnack’s formula requires only 1°39 per cent. ; 
and as this was one of the main points on which Harnack based his 
formula, it is well to consider it. Our lowest result on sulphur is 1°69 
per cent., and as the other three show a close agreement, it is proba- 
ble that the former is somewhat too low. The average of our results, 
however, is but 0:04 per cent. higher than found by Lieberkiihn. O. 
Loewt has recently considered this question, and he found on deter- 
mining the sulphur in coagulated egg-albumin by a modification of 
Piria and Schiff’s method, 1°70 and 1°87 per cent. of sulphur respect- 
ively. O. Nasse,t{ likewise, found in coagulated albumin a content of 
1:72 per cent. of sulphur, and lastly, Hammarsten§ found in non- 
coagulated albumin 1°93 per cent. of sulphur. There would seem to 
be plenty of confirmatory evidence, therefore, that the content of 
sulphur in egg-albumin is much larger than indicated by Harnack’s 
formula. 
The nitrogen, as determined in our preparations, is seen to be 
somewhat higher than found by Hammarsten, with whose results in 
other respects ours most closely correspond. Dumas, however, 
found nearly the same percentage of nitrogen as contained in our 
preparations. The accompanying table of analyses shows the aver- 
age of our results, compared with those of others. 
Albumoses. 
Three distinct digestions of albumin (preparations A, B and C) 
were made with pepsin-hydrochloric acid, and the albumose bodies 
isolated. In this way it was possible to prepare the bodies under 
somewhat different conditions, and to notice the influence, if any, on 
the nature and composition of the products. The pepsin-hydrochloric 
acid used in two of the digestions was prepared with a special view 
* Zeitschrift fiir Physiolog. Chemie, Band v, p. 207. 
+ Pfliiger’s Archiv fiir Physiologie, Band xxxi, p. 395. 
+ Jahresbericht fiir Thierchemie, 1873, p. 13. § Ibid, 1881, p. 19. 
