Chittenden and Bolton—Egg-Albumin and Albumoses. 341 
to removing all traces of albumose bodies, formed by the self-digestion 
of the mucous membrane, and was prepared as follows: 700 grams of 
mucous membrane from the cardiac portion of six pigs’ stomachs, 
freed from the muscularis, were finely divided and warmed at 40° C. 
for fourteen days, in two and a half litres of 0°5 per cent. hydro- 
chloric acid. At the end of this time, all albumose bodies presumably 
having been converted into peptone, the solution was filtered from 
the residue of nuclein, antialbumid, etc., and the filtrate saturated 
with ammonium sulphate. The precipitate, consisting mainly of 
pepsin, with perhaps some albumose, was filtered off, washed with a 
saturated solution of ammonium sulphate, and then dissolved in two 
litres of 0:2 per cent. hydrochloric acid. The acid solution was then 
thymolized and dialyzed in running water, until the ammonium sul- 
phate was entirely removed. On opening the dialyzing tubes, quite 
a precipitate was found, which on being dissolved in 0*2 per cent. 
hydrochloric acid showed marked proteolytic action. The filtrate 
also, on being acidified, showed vigorous digestive power. These 
two solutions of purified pepsin were used in the digestion of two of 
the albumins, while with the third a pure glycerin extract of pepsin 
was employed. 
The general method of procedure, both in the digestions them- 
selves and in the separation of the various albumoses, was much the 
same as that previously employed by Kiihne and Chittenden. 
Digestion of Albumin A. 
The albumin, as previously described, was placed in four litres of 
0-4 per cent. hydrochloric acid and the mixture raised to a tempera- 
ture of 45° C. Then 600 c¢. c. of the purified pepsin-hydrochloric 
acid solution were added and the mixture kept at a temperature of 
45° C. for three hours, after which it was neutralized with sodium 
hydroxide and filtered. The pepsin solution, although quite active, 
did not act very vigorously on the coagulated albumin. The neu- 
tralization precipitate, therefore, together with the unaltered albumin, 
was again treated with a fresh quantity of the pepsin-hydrochloric 
acid, under like conditions as the preceding, for four hours. ‘The two 
neutralized fluids were then united and treated together. The total 
volume was about six litres. The clear fluid was saturated in the 
cold with crystals of sodium chloride, by which a precipitate was 
obtained, which from analogy should consist of proto-, dys- and 
heteroalbumose, 
