342 Chittenden and Bolton—Egg-Albumin and Albumoses. 
In making these separations of the albumose bodies, we intention- 
ally avoided raising the temperature of the fluid above 45° C., for 
fear that heat might induce some change in the character of the 
bodies; hence the first neutralized fluid was saturated directly with 
salt, in spite of its large volume, and the bodies were ultimately all 
separated without having been exposed to a temperature higher than 
that above-mentioned. The use, however, of such a large quantity 
of rock salt introduced into the solutions some calcium sulphate, 
which adhered very tenaciously to the albumose bodies and thus 
unavoidably raised the content of ash in the preparations. 
The precipitate produced by the addition of sodium chloride in 
substance was filtered, washed with a saturated solution of sodium 
chloride, then extracted successively with a ten per cent. solution of 
sodium chloride, a five per cent. solution of the same salt, and lastly 
with water. The residue remaining undissolved after these succes- 
sive treatments with dilute salt solutions and water, presumably con- 
sisted of dysalbumose, while the solutions contained a body precipi- 
table by acetic acid and soluble in excess, and also precipitable by 
potassium ferrocyanide; presumably protoalbumose together with 
heteroalbumose. The original salt-saturated filtrate contained all of 
the deuteroalbumose, together with considerable protoalbumose and 
some heteroalbumose. 
A. Protoalbumose. 
The five and ten per cent. sodium chloride solutions of the first salt 
precipitate, together with the aqueous solution of the same, were 
united and then dialyzed in running water for removal of the hetero- 
albumose. The solution, partially freed from the latter, was concen- 
trated somewhat and the protoalbumose again precipitated by satu- 
rating the solution with sodium chloride. This precipitate was again 
dissolved in water, dialyzed until the greater portion of the salt was 
removed, the solution then concentrated and the albumose precipi- 
tated by alcohol. This precipitate was redissolved in water, dialyzed 
until no chlorine reaction could be obtained with silver nitrate, the 
solution concentrated on the water bath to a syrup and finally pre- 
cipitated with alcohol, washed with alcohol and ether and then dried 
at 106° C. in vacuo until of constant weight. In the last dialysis, 
there was no separation whatever of heteroalbumose, hence the pro- 
toalbumose is to be considered as quite pure. The composition of 
the substance is shown in the accompanying table, The ash con- 
tained no sulphate. 
a 
