Chittenden and Bolton—Kgg-Albumin and Albumoses. 345 
and plumbic acetate, there was a decided blackening of the fluid. 
The protoalbumose likewise gave the characteristic reddish violet 
color with potassium hydroxide and cupric sulphate. Cupric sul- 
phate alone, added to an aqueous solution of protoalbumose, gave a 
heavy greenish colored precipitate, not very soluble in excess of the 
copper salt. Mercurie chloride and lead acetate also precipitated 
the albumose. 
In its reactions, therefore, the protoalbumose formed from egg- 
albumin does not differ, essentially at least, from fibrin protoal- 
bumose. 
A. Deuteroalbumose. 
This body was obtained from the first salt-saturated fluid, by the 
addition of a little acetic acid (sp. gr. 1042) also saturated with salt. 
As Kiihne and Chittenden have already pointed out, all of the proto- 
albumose is not precipitated by saturation of a neutral fluid with 
sodium chloride. Hence, it is to be expected that the deuteroalbu- 
mose solution would contain some protoalbumose, which latter would 
be likewise precipitated by the salt-saturated acetic acid. We en- 
deavored to make a separation, however, by rejecting altogether the 
first precipitate produced by the addition of a little acetic acid, and 
then to obtain the deuteroalbumose fairly free from the former, by 
the subsequent addition of more acetic acid. The final precipitate 
so obtained, was dissolved in a small amount of water and then 
dialyzed for several days. The solution, in which was noticed a small 
deposit of heteroalbumose, was concentrated and finally precipitated 
by alcohol. The precipitated deuteroalbumose was then redissolved 
in water, the solution made exactly neutral with sodium carbonate 
and dialyzed in running water for many days, after which the 
solution was concentrated to a syrup, the albumose precipitated with 
alcohol and finally, after washing with ether, dried at 106° C. in 
vacuo until of constant weight. The composition of the product is 
shown in the accompanying table. The ash was composed mainly of 
ferric oxide and calcium phosphate ; it contained no sulphate. 
The pure white powder, after being dried at 106° C, was found 
readily soluble in water. The solution was not rendered at all turbid 
by saturation with sodium chloride, but the substance was more or 
less completely precipitated by the addition of a little acetic acid to 
the salt-saturated fluid. Nitric acid added to an aqueous solution of 
the substance gaye no precipitate whatever, but colored the solution 
decidedly yellow even in the cold. A little sodium chloride added to 
Trans. Conn. Acap., Vou. VII. 44: Nov., 1886. 
