Chittenden and Bolton—Egqg-Albumin and Albumoses. 347 
the nitric acid solution gave a decided turbidity, which disappeared 
on warming the solution and reappeared on cooling. 
The addition of acetic acid to an aqueous solution of the albumose 
gave no precipitate whatever, nor was any change to be observed on 
heating the fluid; neutralization, at least, caused no precipitation. 
The addition of a little sodium chloride solution to a solution of 
deuteroalbumose acidified slightly with acetic acid gave no precipi- 
tate whatever, but as with deuteroalbumose from fibrin, the applica- 
tion of a little heat induced a slight turbidity, which disappeared on 
raising the temperature still higher. Again, on the further addition 
of sodium chloride, a heavier precipitate was produced which disap- 
peared completely on heating the solution and reappeared on cooling; 
and lastly, by adding more sodium chloride, a precipitate was ob- 
tained which was permanent even on heating the mixture to boiling. © 
In these, as in nearly all other respects, the deuteroalbumose showed 
itself the same in nature as the deuteroalbumose from fibrin, and the 
reactions given for that body can well be applied here. In one reac- 
tion only was there any very noticeable difference; viz: in the 
reaction with cupric sulphate. Deuteroalbumose from egg-albumin 
gave only a slight precipitate with cupric sulphate, even on the addi- 
tion of a minimum amount of the copper salt.* With acetic acid and 
potassium ferrocyanide, the reaction was much the same as with pro- 
toalbumose. Boiling with sodium hydroxide and lead acetate gave 
a decided blackening of the fluid, from the presence of sulphur. 
A, Heteroalbumose. 
The greater portion of the heteroalbumose was obtained by the 
dialysis of the 5 and 10 per cent. sodium chloride solutions of the 
first salt precipitate, viz: in the purification of protoalbumose. 
Some, too, was also found in the dialysis of the precipitated deuteroal- 
bumose. In both cases, the albumose was left as a more or less gummy 
precipitate, closely adherent to the parchment of the dialyser, sepa- 
rating out as the sodium chloride left the solution. The product 
was purified by solution in 5 per cent. sodium chloride, re-precipitation 
by the addition of salt in substance, re-solution in 5 per cent. sodium 
chloride and separation by dialysis, continued until all chlorine was 
* This fact simply shows the greater purity of this preparation of deuteroalbumose 
or rather its freedom from protoalbumose, for as Dr. Neumeister has recently shown, 
perfectly pure deuteroalbumose gives no precipitate whatever with cupric sulphate. 
Later, we were able to prepare deuteroalbumose entirely free from protoalbumose. 
