Chittenden and Bolton—Egg-Albumin and Albumoses. 349 
removed from the solution. Like heteroalbumose from fibrin, this 
product each time it was re-dissolved in dilute sodium chloride, left 
a residue soluble only in dilute acids; presumably dysalbumose. 
After being washed with water, alcohol and ether, the product 
was dried at 106° C. 7m vacuo and then analyzed with the results 
shown in the accompanying table. 
The ash consisted mainly of calcium phosphate and a little ferric 
oxide, but did not contain any sulphate. The reactions of the body 
were found to be almost identical with those described as character- 
istic of heteroalbumose from fibrin.* Suspended in water or dis- 
solved in 5-10 per cent. sodium chloride solution and then heated to 
boiling, the heteroalbumose was changed into a body, coagulated 
heteroalbumose, insoluble in sodium chloride but slowly soluble in 0°2 
per cent. hydrochloric acid, from which it was precipitated by neu- 
tralization, apparently reconverted again, in part, into soluble hetero- 
albumose and in part into a body resembling dysalbumose. Thus 
on neutralizing the acid solution, a decided precipitate was obtained 
which was in part soluble in 5 per cent. sodium chloride (heteroalbu- 
mose), while the residue insoluble in the salt solution was soluble in 
dilute acids and in dilute alkalies (dysalbumose). In the filtrate 
from the neutralization precipitate, acetic acid showed the presence 
of still more heteroalbumose, which could be separated from the 
solution by dialysis. 
Further, the heteroalbumose was found to be soluble in dilute 
acids, alkalies and alkaline carbonates, and from the solutions thus 
formed, it was reprecipitated by neutralization, but never completely ; 
the amount remaining in solution being dependent naturally on the 
amount of neutral salt contained in the fluid. 
Nitric acid precipitated the albumose, the extent of the precipita- 
tion depending on the amount of sodium chloride present. Acetic 
acid likewise gave a precipitate, soluble in excess of the acid. With 
acetic acid and potassium ferrocyanide, a precipitate was formed also 
soluble in excess of acid. With cupric sulphate, on the contrary, 
a heavy precipitate was obtained in a sodium chloride solution of the 
albumose, insoluble in excess of the copper salt. The substance gave 
the so-called biuret reaction with cupric sulphate and potassium 
hydroxide quite plainly, and also gave evidence of the presence of 
sulphur on boiling with potassium hydroxide and plumbic acetate. 
* See Zeitschrift fiir Biologie, Band xx, p. 32-36. 
