Chittenden and Bolton—Egg-Albumin and Albumoses. 353 
centrated to a syrup and the albumose precipitated by alcohol. This 
precipitate was again dissolved in water, the solution made exactly 
neutral and again dialyzed. 
After suitable concentration, the albumose was again precipitated 
by alcohol, washed with alcohol and ether and finally dried at 106° C. 
in vacuo. 
The results of the analysis are seen in the accompanying table. 
The ash in this preparation is seen to be much smaller than in the 
protoalbumose precipitated by salt alone. In other respects the two 
analyses are closely comparable, particularly the carbon and sulphur. 
The reactions were in almost every case the same as with the pre- 
ceding preparation, excepting perhaps a somewhat greater solu- 
bility. 
B. Deuteroalbumose. 
This body was separated and purified in exactly the same manner 
as in the preceding digestion. The analysis of the product is shown 
in the accompanying table. The ash contained some calcium sul- 
phate and a little ferric oxide. The reactions of the body were the 
same as those of A deuteroalbumose. 
From this digestion, more or less heteroalbumose was separated 
but no analysis was made of the product, as the amount was rather 
small for the necessary purification. 
Digestion of Albumin C. 
In the digestion of this sample of coagulated albumin, a much 
more vigorous pepsin-hydrochloric acid was employed than in the 
preceding digestions. The freshly coagulated albumin was placed 
in 3 litres of 0°4 per cent. hydrochloric acid and brought to a tem- 
perature of 45° C., then 400 c.c. of a pepsin solution, made from a 
pure glycerin extract of pepsin, were added and the mixture kept at 
45° C. for 24 hours. The fluid was then neutralized, filtered and the 
clear filtrate saturated with sodium chloride. The albumose bodies 
were then separated and purified according to the methods already 
described. 
The several bodies showed the same reactions as observed in the 
preceding preparations. 
Protoalbumose and deuteroalbumose were analyzed. ‘The results 
are shown in the accompanying tables. The ash of the deutero- 
albumose contained no sulphate, but was composed almost entirely 
of ferric oxide. 
TRANS. Conn. ACAD., Vou. VII. 45 Nov., 1886. 
