Primary Cleavage Products. 381 
saturated fluid and was thrown down as a white curdy precipitate 
by the addition of a little acetic acid, evidently some protocaseose 
not precipitated by salt alone. The main precipitate of protocaseose, 
ete., twice precipitated by salt, was treated with 3 litres of 10 per 
cent. salt solution, the residue with 3 litres of 5 per cent. salt solution 
and the residue still remaining, with 3 litres of water. In this man- 
ner, all of the proto and heterocaseose was dissolved, leaving a 
small residue wholly insoluble in dilute salt solutions and in water; 
presumably dyscaseose. The latter, however, was in exceedingly 
small quantity. It was dissolved in 0:2 per cent. hydrochloric acid 
and reprecipitated by neutralization of the solution with sodium 
. carbonate. 
A. Protocaseose. 
The 5 and 10 per cent. salt solutions of protocaseose together with 
the aqueous solution, were united and the mixture saturated with 
sodium chloride. Here, as before, all of the protocaseose was not 
precipitated ; a portion remained in the filtrate and was precipitated 
only on the addition of a little acetic acid. The main portion of the 
protocaseose precipitated for the third time with salt in substance, 
was dissolved in water, the solution filtered and divided into two 
parts. One part was thymolized and dialyzed in running water 
until all chlorine was removed from the solution (Protocaseose A 1). 
The other part was again saturated with salt, the precipitate washed 
with saturated salt solution, then dissolved in water and like the 
former dialyzed until all chlorine was removed from the solution 
(Protocaseose A 2). In protocaseose 2, there was more evidence of 
the presence of a body resembling heteroalbumose than in No. 1. 
Thus in No. 2, quite a little gummy substance separated from the 
solution on dialysis, but the amount even here was not large. When 
the dialysis was finished, both solutions were perfectly neutral to 
test papers and in both cases the protocaseose was separated from 
the clear fluid by evaporation and precipitation with alcohol. For 
analysis, both products were washed thoroughly with alcohol and 
ether and finally dried at 105° C. in vacuo. 
Their composition is shown in the accompanying tables. 
As already stated, every time protocaseose was dissolved in water 
and reprecipitated by saturating the solution with sodium chloride, 
a certain amount of the substance remained in solution, precipitable 
only on addition of a little acetic acid. Protocaseose precipi- 
tated from the salt-saturated solution in this manner by acetic acid, 
