Primary Cleavage Products. 401 
Analysis of the dried substance gave the following results : 
I. 0-4011 gram substance gave 0°2463 gram H.O=6°82 per cent. H and 
0°7434 gram CO.=50-54 per cent. C. 
_ II. 0°4287 gram gave 53°6 c.c. N at 18°6° C. and 760°1 mm. pressure 
=14-70 per cent. N. 
Ill. 0:4118 gram gave 0:0256 gram ash =6:21 per cent. 
The ash-free substance would therefore contain 
- 53°88 per cent. C, 7°27 per cent. H, 15:67 per cent. N, 
Reactions of the caseoses. 
Under this head little need be said. The reactions characteristic 
of the albumose bodies in general will apply here. Certain differ- 
ences, however, have already appeared in our description of the 
processes incident to separation of the caseoses.. Protocaseose, 
unlike protoalbumose, is precipitated from an aqueous solution by 
acetic acid. The precipitation, however, is not complete; satura- 
tion of the acid filtrate with sodium chloride, invariably gives an 
additional precipitate which is the heavier, of the two. Further, 
long-continued washing of the acid precipitate with water or salt 
solution appears to partially remove the acid from the caseose body. 
Protocaseose is likewise precipitated from an aqueous solution by 
hydrochloric, nitric and sulphuric acids; the precipitate, however, is 
far less soluble in excess of sulphuric or nitric acid than in excess of 
the other two acids. In very dilute acids, protocaseose is soluble 
and is partially precipitated by addition of stronger acid of the 
same kind; ¢hus the substance is readily soluble in 0°4 per cent. 
hydrochloric acid, from which solution it is precipitated by the addi- 
tion of a little concentrated acid, this precipitate dissolving on the 
addition of more acid. Evidently then, protocaseose as fast as 
formed by the action of pepsin-hydrochloric acid, would dissolve in 
the acid gastric juice and not be mixed with the jelly-like insoluble 
residue. Boiled with dilute or strong acid, protocaseose is appar- 
ently not changed; at least no precipitate is obtained on neutraliza- 
tion of the acid fluid. The acetic acid solution of protocaseose gives 
avery heavy precipitate with potassium ferrocyanide. In an aque- 
ous solution of the substance, cupric sulphate gives a heavy curd-like 
precipitate, while ferric chloride gives a similar precipitate read- 
ily soluble in excess of the precipitant. Like protoalbumose, proto- 
caseose is precipitated by saturation of its aqueous solution with 
sodium chloride, but never completely ; there always remains in the 
Trans. Conn. AcaD., VoL. VII. 51 MARCH, 1887. 
