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Transactions of the Canadian Institute. 



[Vol. VII. 



change necessary occasionally, owing to the very rapid growth of algae 

 in the solution, which, if not removed, might in a short time become a 

 hot-bed of bacteria injurious to the young buds upon the twig. 



Observations were made and results recorded upon five different 

 times during the course of the experiment which lasted over a period of 

 forty-three days. 



On March 19th, five days after the apparatus was set up, it was 

 found that upon (a) there were growing buds between the two vessels 



FIG. 16. 

 G and H contain water or solution as required. A, C, E, bottles with bottoms removed and upturned 

 to hold liquid, cork being made so as to prevent liquid from running out. K, is a large " T" tube filled 

 with water through which a twig passes. 



but none in either liquid. Twig (b) had one growing bud in the upper 

 liquid but no others growing. Twig (c) had none at all. 



On March 26th, twig (a) had no growing buds in the upper liquid 

 but had very healthy buds between the jars ; twig (b) had growing 

 buds between the jars and the two in the upper solution were also 



