342 Transactions of the Canadian Institute. [Vol. VII. 



lower vessel and nutrient solution in the upper (two buds in the liquids 

 in each case). 



March 19th, we found small buds developed, f«//J above the upper 

 liquid in (d) ; in (e) no buds developed yet. 



March 26th, (d), all the buds above the upper liquid were developed- 

 (e) small buds developed in the upper solution which was somewhat 

 green owing to a growth of algae which seemed to thrive vigorously in 

 the solution used. 



April 8th, (d), both buds developing in the upper solution and some 

 above but none below ; (e) both buds in the upper solution growing, 

 several above and one below were growing. 



April 23rd, (d), leaves all living above the upper solution ; (e), 

 leaves all dried above the upper solution and below it, but not in the 

 upper liquid. 



The one important difference between this experiment and the 

 preceding one is that there was no development of roots in the upper 

 liquid, and almost no development of leaves between the jars where 

 there was a vigorous growth of leaves in Series I. 



At the conclusion of the experiment, upon examining the root 

 systems of the twigs, it was found that (d) had a healthier root system 

 than (e). This must have been due to the difference of conditions, 

 which were, that there was a nutrient solution in the upper jar in the 

 one case, and water in the other, the nutrient solution above, having 

 apparently entered through the buds and penetrated as far as the roots. 



Series III. was arranged on the same date as the others, and 

 according to the plan shown in Fig. 16, 5 ; (f) was in a nutrient 

 solution ; (g) in distilled water ; and (h) in tap water. (Two buds in 

 each liquid, and several others besides). 



March 19th, the roots of (f) were most numerous but short, and the 

 buds of all three twigs were commencing to develop ; little if any 

 difference between (g) and (h). 



April 8th, the tips of the buds of (f) were dead, while the buds of the 

 others were living. 



April 23rd, the leaves of (f) were all withered and the twig appeared 

 dead. Both the others were living. The root systems were compared 

 and it was found that the roots of (f) were rather numerous but stunted 



