466 Transactions of the Canadian Institute. [Vol. VII. 



Dr. John Waddell and Mr. C. W. Dickson, M. A., both of the School 

 of Mining, Kingston, rendered valuable aid in determining the amount 

 of solid matter in sawdust water. 



The Ontario Fisheries Department facilitated my task on the Bon- 

 nechere by instructing their overseers to assist me in every way possible. 



APPENDIX TO DR. KNIGHT'S REPORT ON SAWDUST 



AND FISH LIFE. 



Bacteriological Examination of Sawdust Water in Shade 

 and in Sunshine. 



Ex.amination of sawdust water in aquarium made July 31st, 1902. 



Two agar plates made. The /frj/ averaged 3,300 colonies of bacteria per cubic centi- 

 metre. None of the colonies were spirilla which were present in large num- 

 bers in direct microscopic examination of the water. The chief colonies were 

 those of a spore bearing bacillus, a variety evidently of B. Subtilis ; also a few 

 sarcinae, particularly one like Sarcina Lutea. The second plate averaged 3,570 

 colonies per cubic centimetre. In general characters they were the same as in the 

 first plate. 



August 4TH, 1902. Water in aquarium. Agar plates averaged 3,570 colonies per cubic 

 centimetre. These were in all respects like those of July 31st. 



Sat?ie water in simlight since July jist. Agar plates average 4,200 colonies per cubic 

 centimetre. These colonies contain the same bacteria as in the aquarium water, 

 but in fewer numbers. Further, there is present a fluorescent bacillus, making up 

 half the number of colonies present. - .: 



August 8th, 1902. Water in aquarium. Agar plates develop 7,870 colonies per cubic 

 centimetre. These colonies are of the same type as those found on previous plates 

 with the addition of about 1,000 colonies of B. Mesentericus Vulgatus per cubic 

 centimetre. 



Water in sunlight. Agar plates develop 37,070 colonies per cubic centimetre. These 

 consist mainly of B. iFluorescens Liquescens ; also of Sarcina Lutea, and an occa- 

 sional colony of B. Subtilis. 



W. T. CONNELL, 



Prof, of Bacteriology. 



