504 Transactions of the Canadian Institute. [Voi.. VII. 



GLIADIX NUCLEIX. 



C 49-47 P<s'' cent. Av. 



H 6.q8 



N 16.60 



S 0.80 



P 0.29 



Fe 0.04 



O 25.82 



Av. 2. 

 Av. 2. 

 One. 

 Av. 2. 

 Av. 2. 



Ash 0.24 per cent. Av. 2. 



The amount of phosphorus was very small, practically the same, 

 in fact, as the gliadin from which it was prepared. The chemicals used 

 were carefully tested in blank experiments, but no trace of phosphorus 

 was found in any of them. Possibly the prolonged digestion with 

 frequent renewals of hydrochloric acid solution had removed some of 

 the phosphorus. The result was, however, quite unsatisfactory, since it 

 was to be expected that the amount of phosphorus and iron would be 

 much greater than in the substance from which it was derived. The 

 analyses of the gliadin and the nuclein, derived from it, may be com- 

 pared side by side : — 



Gliadin. Gliadin Nuclein. 



C 52.39 49-47 • 



H 6.84 6.98 



N '7-47 16.60 



S 1. 1 2 0.80 



O 21.89 25.82 



P 0.267 0.29 



Fe 0.034 0.04 



100.00 100.00 



From this it may be gathered that the two compounds are quite 

 distinct chemically as well as physically. 



Glutenin was prepared, as recommended by Osborne and Voor- 

 hees^^ by extracting all the gliadin from gluten by dilute alcohol, 

 dissolving the residue in 0.2 per cent, potassic hydrate, and precipitating 

 by exactly neutralizing with 0.2 per cent, hydrochloric acid ; the pre- 

 cipitate washed with 70-95 per cent, alcohol, was again dissolved in 0.2 

 per cent, potassic hydrate and filtered perfectly clear through heavy 

 filter paper in an ice chest. Precipitated from the solution by exact 

 neutralization with 0.2 per cent, hydrochloric acid, washed with distilled 

 water till free from chlorides, then with .70-95 per cent, alcohol, extracted 



