22 SUMMA KY. 



The experiment of gerniinating the spores after a periocJ of rest did not 

 succeed at all; equally unsuccessful was an experiment with the spores of some 

 toadstools eaten by snails which according to the investigations of Voglino 

 (nota micologica 1891) will only germinate after having passed through the 

 stomach of these animals. 



Notwithstanding these negative results, the writer does not believe, that 

 all these beautiful earth-living-toadstools are incapable of reproduction by theii' 

 spores. She only thinks that man can not imitate the conditions under which 

 they germinate in Nature. 



She begs her mycological colleagues to follow up her gerraination-investigations. 

 It gives little trouble and it is very interesting to see the different modes of 

 gerniinating in the different species and when many people experiment on the 

 same subject, perhaps one of them discover the conditions of success. In her 

 investigations the writer tried to make pure cultures from species which possess 

 germinating spores. For this purpose she placed the cap of the freshly gathered 

 toadstool (facing downwards) on a wadding ring, which is placed on a sterilized 

 glassplate or Petri dish. After some hours enough spores have fallen from the 

 cap to be sown; this she did, by the so called: „Bacteria pure culture method", 

 and with a nutriënt substratum of cherry-agar. 



From the 66 species sown, the writer obtained 22 purecultures, belonging 

 for the greater part to the woodgrowing toadstools. Of these 22 cultures 

 15 species, that is 67 % give more or less normal fructifications. The first 

 fructification to appear (5 weeks after sowing and after growing the mycelium 

 on steriUzed wood in a bottora of wet sand, was: Collybia velutipeè (fig. 10). 



Polyporus versicolor gives already fructification, IS days after sowing on 

 cherry-agar in Petridish. 



As the writer could not obtain pure cultures by sowing the spores of the 

 beautiful earth-toadstools, she adopted for them the so called ,^tissue culture 

 method" described by O. Brefeld. A small piece of tissue from stem or cap was 

 therefore cultivated on a nutriënt substratum, in this case also cherry-agar. 



By this method she succeeded with the earth-species as well as with the 

 wood growing species, but only with those former which are thick-fleshy. 



In general the toadstools are too watery for this method of culture and 

 most of them make absolutely no growth in tissue-cultures. From the 150 species 

 which the writer experimented with, only 20 that is 13% give pure cultures 

 and 6 from those that is 30% have produced fruits. It is to be remarked, that 

 most of the cultures, the names of which are given on page 16 and 17, are 

 still very young and may fnictify before long. 



Generally the temperature in which the myielium of all these pure cultures 

 grew best, was from 16— 20o C. and that when kept from daylight. 



To produce the most normal fructifications the temperature must be below 

 160 (12— ir.0) and dayhght, even sunhght, must be allowed. 



