Tank. 



3 



4 



5 



6 



7 



8 



9 



lo 



II 



12 



13 upper 

 13 lower . 



14 



15 



16 



CARCINOMA OF THE THYROID IN SALMONOID FISHES. 

 Table VIII.— Feeding and Other Experiments with Wild Brook Trout. 



461 



Food. 



Heart, raw. . . 



Liver, raw. . . 



Marine fish.. . 



Liver, raw. . . 



Vegetable. . . . 

 do 



Liver, cooked 



Liver, raw. . . 



Natural food. 



Liver, raw. . . 



Natural food . 



do 



do 



....do 



.do., 

 .do.. 



Other conditions. 



Reduced space; crowding. . 



In presence of tumor fish. , 



In presence of tumor fish , 



Minimum feeding 



Forced feeding , 



Controls 



Pond 10 mud in tank 



Controls for tanks 12-16 



Inoculated in thyroid region 

 with trout thyroid tumor. 



Pond 10 mud in stomachs 



Inoculated in thyroid region 

 with trout thyroid tumor. 



Inoculated behind eye with fil- 

 trate from pond 10 mud. 



Fish 



added 



Sept. 21, 



1910. 



Clinical condition, June, igia." 



Remain- 

 ing. 



r 20 



38 



40 



Clean. 



With red 

 floors. 



With tu- 

 mors. 



Sec- 

 tions, 

 igii. 



Diagnoses, 1911. 



Seo 

 tions, 



1912, 



Diagnoses, 191 2. 



3 



4 



5 



6 



7 



8 



9 



10 



n 



12 



13 upper 

 13 lower . 



14 



IS 



16 



?ooo 

 2001 



3003 

 2003 

 2CX>4 

 2005 

 2006 

 2007 

 200S 

 2009 

 2010 

 201 1 

 3012 

 2013 

 2014 

 201s 

 2016 



A, hyperplasia; B, carcinoma 



A, beginning hyperplasia; B, normal; C, carci- 

 noma. 



Normal 



Beginning carcinoma 



A, B, C, normal 



Normal 



A. B, normal 



A, B, besinning hyperplasia 



A. B, C, normal 



A, B, normal 



B, C, normal 



A, B, C, normal 



A, B, normal 



A, B, normal 



Normal 



A. normal; B, C, beginning hyperplasia 



Normal 



2098 

 2099 



3IOO 



2101 

 ai02 



2103 

 2104 

 2113 

 2105 

 2106 

 2107 

 2108 

 2109 

 2110 

 21J1 

 2112 



A, tumor, carcinoma; B, adeno-carcinozna. 

 A, B, C, regression. 



Normal. 



Regression. 



Normal. 



Regression. 



A, B. regression. 



Normal. 



Regression. 



Normal. 



Increased thyroid of normal type. 



Increased thyroid of normal type. 



Normal. 



A, normal; B. increased thyroid of normal type. 



Increased thyroid of normal type. 



Increased thyroid of normal type. 



o Examinations of Nov. 30. 1910, and July, 1911, all were clean. 



6 Transferred to pond 10. 



DESCRIPTION OF SECTIONS TO ACCOMPANY TABLE VIII. 



2000 B. Tubulo-alveolar type of carcinoma. Marked infiltration of surrounding areolar tissue. Infiltration of muscle above 

 into bone spaces and infiltration of vessel wall. Invasion of cartilage. 



2001 A. Beginning hyperplastic stage. Certain follicles are lined with columnar epithehum. In many such follicles colloid is 

 absent, in others present but poorly stained. Surrounding this group of follicles are follicles of strictly normal appearance. Epi- 

 thelium flattened, filled with colloid. Beginning of the hyperplasia in this specimen is evidently limited to individual follicles 

 (Fig. 84.) 



2001 B. Most of the thyroid tissue in this specimen is of typical normal appearance, flattened epithelium, follicles filled with 

 stainable colloid. 



2001 C. The entire space surrounding the vessel is filled with closely packed alveoli, small sized alveolar structures lined with 

 high columnar epithelium, with deeply staining vesicular nuclei. Colloid is almost entirely absent. Evidently growth of the 

 thyroid tissue which has spread upward into the areolar tissue immediately below themucosa of the floor of themouth. Under 

 high power the nuclei present great variability in size, the staining qualities are vesicular with one or two nucleoli. Marked 

 evidence of hyperemia between the follicles. Many tubules are filled with closely packed cells. In some portions of the 

 growth all alveolar structure has disappeared and small islands of closely packed cells are found. Infiltration of dense con- 

 nective tissue structure, perichondrium and periosteum. Beginning carcinoma of tubulo-alveolar type. 



