38J 



not shown in carmine stainings, but are readily demonstrated 

 in fresh preparations by the action of solutions of osmic acid. 



or of chloride of gold. Indications of the muscular bands 

 formed by the union of these fibre-cells are, however, seen in 

 the photograph, particularly on each side of the principal 

 venous trunk. The second variety of nuclei are oval, about 

 1 -3000th of an inch long, and belong to the connective tissue 

 of the bladder. The cells in which these nuclei lie are not 

 seen, the action of the carmine being limited to the nuclei. 

 They can, however, readily be demonstrated in fresh prepara- 

 tions by gold-chloride and some other reagents. The cut 

 represents the outlines of a portion of the photograph ; a, a, 

 are the nuclei of the vascular epithelium ; b, b, the nuclei 

 of the muscular fibre-cells ; c, c, those of the connective tissue. 

 II. Photograph representing a small vein from another 

 portion of the same preparation. Negative No. 195, New 

 Series. Magnified 1000 diameters by Powell and Lealand's 

 immersion 1-1 6th objective, illuminated by the Magnesium 

 lamp. One of the epithelial cells near the centre of the vessel 

 is particularly well defined, and shows its nucleus handsomely ; 

 the adjacent cells, not lying in the same plain, are many of 

 them partly out of focus, but their boundaries can readily be 

 traced, and the nuclei of several of them are well defined. 



