248 TRANSACTIONS OF THE CANADIAN INSTITCTE. [VoL. T. 



with division 3, the treatment of whicli I postpone until I have finished 

 my experiments on the methods of the resorption of chromatins (Nu- 

 deins). 



To illustrate the parasitic nature of some of these forms, I will now 

 describe undoubted examples of intracellular parasites from the intestines 

 of the spotted newt and the lake lizard (Necturns). 



I. A Cellular Parasite from the Intestinal Epithelium of 



DiEMYCTYLUS ViRIDESCENS. 



In April of this year I obtained from the neighborhood of Toronto a 

 number of spotted newts for the purpose of studying the phenomena of 

 secretion in the pancreas and in making preparations of this organ I 

 found it frequently convenient, on account of the small size of the animal 

 and its organs, to include the anterior portion of the intestine. In the in- 

 testinal epithelium of one of the newts was found a large number of 

 forms like those shown in Figures 3 and 4, and I immediately endeavored 

 to work out their history. Before detailing the results of this work it 

 may be well to state that the particular object from which the sections 

 studied were made was hardened in Flemming's Fluid and alcohol, stained 

 in toto with haematoxylin, imbedded by the chloroform process in paraffin, 

 the sections therefrom placed in series on the slide and stained with eosin 

 and safranin, before being permanently mounted in balsam. 



The structures in question are so numerous that every second or third 

 epithelial cell, for long stretches of the section, contained one of them. They 

 are always placed in the outer half of the cell between the nucleus and the 

 free border, and have a nearly uniform diameter (9-1 i/x, averaging io/a) 

 and an approximately spherical shape. They do not appear to have a 

 definite or distinct membrane, and what takes its place appears to be a 

 zone of homogeneous or faintly granular protoplasm which, in many 

 cases, is denser and thicker at one side of the body than at any other. 

 From this zone trabeculae of granular protoplasm pass inwards to ter- 

 minate in a more or less centrally placed protoplasmic mass. In a 

 number of these bodies sufficient to render the peculiarity prominent, the 

 bulk of the protoplasm is collected at one side (Fig. 3), while the thicker 

 portion of the protoplasmic rim occupies the opposite side with a large 

 crescentic, oval, or round cavity intervening. The protoplasmic mass 

 stains lightly but readily with eosin and contains a round homo- 

 geneous nuclear body, which stains deeply with safranin and measures less 

 than 2/A (i-S/^)- Sometimes the nuclear body is placed in a cavity in 

 the protoplasmic mass and connected with the latter by a i&\^ fine 

 strands. In a few instances, the nucleus was surrounded at a distance by 



