266 TRANSACTIONS OF THE CANADIAN INSTITUTE. [VoL. I. 



with haematoxylin less vivid, while the larger bodies disintegrate and the 

 fragments become scattered through the cell. The disappearance of these 

 elements, the concurrent increase in the cell protoplasm and the appear- 

 ance of zymogen granules are not matters of physiological relation. 

 The removal, or rather the disappearance of chromatin, is on the other 

 hand in some way connected with the abundance of chromatin in the 

 greatly enlarged nucleus of the containing cell (Figs. 3, 4, 5, and 6). 

 The nucleus may be somewhat distorted in its shape, and this is without 

 doubt due to the abundance of the chromatin which it has absorbed 

 from the elements in the cell. The processes of disintegration and 

 absorption go on till finally in the resting gland cell a few protoplasmic 

 masses, scarcely larger than zymogen granules, may remain. 



The origin of these bodies is to be sought for in the broken down 

 gland cell. Indeed one can see them so derived in the sections. In 

 Figs. 3 and 5 are some of the remains (cm and ro/ie) of such disintegrated 

 cells lying in the intercellular spaces, while the surrounding cells contain 

 masses, which, from their position, are evidently swallowed portions of the 

 same. The farther a cell is removed from these intercellular masses the 

 freer it is from the intracellular elements in question and at a distance not 

 greater than the diameter of a cell these may be absent altogether. In 

 other words, wherever one finds the intracellular bodies numerous one can 

 also in the same or in the next section find intercellular elements to in- 

 dicate the place of origin of the former. It is quite possible that disin- 

 tegrated leucocytes may give rise to the same, but I have seen no evi- 

 dence of such, except, perhaps, in such forms as that represented in 

 Fig. 6a. 



These bodies are also present more or less in the pancreas of all the 

 young Amblystouiata examined and they exhibit here also the same vary- 

 ing composition and structure. 



These bodies do not participate in the processes of secretion. The 

 presence of eosinophilous granules, like those constituting the zymogen, 

 led Ogata to consider them as breaking up into zymogen and from the 

 fact that the parasites may appear to contain zymogen granules more or less 

 imbedded in them, he concluded that the latter are earlier phases in this 

 formation of zymogen. These eosinophilous granules are not formed of 

 zymogen, however, because in the more centrally placed cells in a sec- 

 tion of the pancreas prepared with Flemming's Fluid, the zymogen gran- 

 ules are dissolved out by the acetic acid in this reagent, but the eosinop- 

 hilous granules are not affected. This phenomenon has a bearing on the 

 mode of secretion and I will, therefore, forego an explanation of it till I 

 come to this subject farther on. 



