STUDY OF COCCIDIA MET WITH IN MICE. 279 



No change was noticed in the animal until six days after the 

 parasites were administered. On this day its coat was noticed 

 to be rough, the abdomen was distended^ and the thighs some- 

 what drawn up towards the belly, so that the animaFs gait 

 was stiff. The stools were softer than normal. The next 

 day blood ^ was noticed about the animal's anus, and this 

 sanguineous discharge contained coccidia of the same dimen- 

 sions as those described above. On the seventh day the animal 

 was distinctly ill and suffering, so it was killed. After death 

 the stomach was found to be distended, the contents consisting 

 chiefly of parasites, many of which showed the formation of 

 sickle-spores^ as shown in fig. 8. The large and small intes- 

 tine contained practically little beside ripe Coccidia. The 

 stomach, intestines, and portions of the liver and kidneys were 

 fixed in saturated solution of corrosive sublimate and hardened 

 in the usual way. After embedding in paraffin, sections were 

 cut and stained in Ehrlich's acid hsematoxylin and eosin 

 (Griibler's wasserloslich). 



Histological Examination. 



In the stomach the glands of the cardiac end contained free 

 Coccidia, many of which were encapsuled and filled with small 

 swarm-spores as shown in fig. 8 ; but the most striking feature 

 consisted in masses of minute bodies exactly resembling the 

 swarm-spores within such capsules, but stained and lying free on 

 the surface of the mucous membrane, and distending the ducts 



coccidia presented the same appearance. On the contrary, up to nineteen 

 days after the parasites were placed in the moist chamber, nearly all the 

 phases could be observed at the same time ; but on the date last named all 

 the parasites were subdivided into sporocysts, each of which comprised two 

 spores and a nucleus de reli([uat, as the residual body has been termed by 

 Aime Schneider. 



^ This phenomenon is of interest in connection with an observation recorded 

 by Hess (' Schweitz Archiv fiir Thier.,' Zurich, 1892, vol. xxxiv, p. 125), to 

 the effect that the " Rothe Ruhr " of cattle is associated with the presence of 

 a coccidium. 



2 Compare E,. Pfeiffer, ' Beitrage zur Protozoon-rorschung,' Berlin, 1892, 



