OBSERVATIONS ON VARIOUS SPOROZOA. 287 



peripherally, and also in spaces extending from the periphery 

 into the remains of the body substance of the parasite. 



The single nucleus of the sporogonia subdivided into eight, 

 and meanwhile the sporogonia became surrounded by capsules 

 constituting sporocysts (pseudo-navicellse), the substance of 

 which split up into eight crescentic spores, each of which 

 contained a nucleus. Such are the chief results obtained by 

 Wolters with regard to the Monocystides, and the thorough- 

 ness of the work and the beautiful drawings by Nussbaum, at 

 whose instigation the work was undertaken, go far to establish 

 the conclusions arrived at. 



I will now detail some of the features I have obtained by 

 examining the seminal vesicles of Lumbricus agricola, 

 taken in the month of May. I have not had the opportunity 

 of making such complete serial sections as Wolters, and in so 

 far my criticism must be incomplete ; still, the observations 

 recorded below may be found of some interest. In this place 

 it is advisable to describe the methods employed. Wolters 

 found, and I have had the same experience, that Flemming's 

 fluid did not give good results with gregarines. Wolters's 

 results are chiefly based on the examination of sections of 

 material fixed in saturated solution of picric acid. I have 

 employed a method more commonly adopted, and which I have 

 found most satisfactory, not only for gregarines, but for 

 Coccidium oviforme and for animal tissues in general. 

 Small portions of the tissue are placed for twenty-four hours 

 in Foa's reagent, i. e. a mixture of equal parts of a saturated 

 solution of corrosive sublimate in normal saline solution and 

 a 5 per cent, solution of bichromate of potassium or Miiller's 

 fluid. Then the material is transferred for twenty-four hours 

 to running water, and afterwards placed on successive days in 

 30, 60, and 90 per cent, alcohol. After that they are placed 

 in absolute alcohol, and after saturation with chloroform are 

 embedded in parafiin, care being taken that the bath does not 

 reach a temperature higher than 50° C. The sections were 

 cut with a Minot's microtome, and fixed on the slide with 

 albumen and glycerine. After the usual process they were 



