OBSERVATIONS ON VARIOUS SPOROZOA. 291 



numerous examples of Helix hortensis. The kidneys of the 

 snails and the slugs were all alike infested by the sporozoa, 

 but only in the slugs did 1 find swarm-sporing side by side 

 with the ordinary mode of reproduction by sporocysts. One 

 of these parasites, as seen in a teased preparation of a slug's 

 kidney, is shown in fig. 4. The nucleus (a) was large and oval, 

 and showed a single large nucleolus {b). In fig. 5 is repre- 

 sented a cell [a] as seen in a section, and containing a parasite 

 subdivided into four sporocysts (c) (others were present out of 

 focus), each of which contains six crescentic spores (d). In 

 some of these a single nucleus could be made out. The spores 

 were very large, averaging 12 jj. in length. All the sections of 

 the kidneys of several slugs showed a marked infection, and 

 in most of the sections swarm-sporing was well seen. Fig. 6 

 shows an example of this. A much hypertrophied cell (a) 

 contains a large sporing parasite, and six smaller parasites (e). 

 The sickles (b) are very large, 20 ju in length. Some of them 

 are undergoing farther subdivision.^ The capsule of the para- 

 site has ruptured, and at the point of rupture are some free 

 sickles, which also are undergoing farther subdivision (c). The 

 appearance of swarm-sporing in a fresh teasing is shown in 

 fig. 7, where some detached sickles [b) are present. The colour 

 reactions in these sections were not good, probably owing to 

 the slugs having in the first instance been placed in Scotch 

 whiskey, so that they will not serve as a basis for comparison 

 with Wolters's descriptions ; but since I have been able to find 

 in Coccidium oviforme all this author encountered in 

 Klossia, and also many additional features, I will pass to the 

 consideration of this more familiar parasite. For examination 

 I chose a highly infected liver in which the lesions were still 

 in process of evolution. Fig. 8 shows an average appearance 

 of a portion of the epithelial lining of a cyst as big as a small 

 pea. All the larger parasites show signs of nuclear activity. 

 The chromatin, in nearly every instance, gave a typical deep 

 blue reaction to acid hsematoxylin. The most abundant form 



* The large sickles formed in swarm-sporing would thus appear to have the 

 equivalents of sporogonia, not of spores. 



