206 LILY H. HUIE. 



gland cells resulted ; but as this alteration might have been 

 due to traces of soluble substances it was thought necessary to 

 use paraflSa of a high melting-point (58° C), which had been 

 carefully cleaned by boiling. Shavings of it were laid on the 

 tentacles, and pressed into thorough contact with them by 

 means of a brush. About one hour after tiie application of 

 the paraffin the alteration in the cells reaches its height. It 

 consists in a vacuolation of the cytoplasm, but with no 

 alteration in colour reactions. The nucleus remains quite 

 unchanged. In the course of twenty-four hours complete 

 recuperation has taken place. 



White of Egg [stains Red] (fig. 3). — My former research 

 having dealt minutely with the changes produced in the gland 

 cells by white of egg, I have now only to add those resulting 

 in the exceedingly short time of five seconds. They are as 

 follows : 



Cell wall very pale blue. 



Cell plasm deep purple, frequently red round the nucleus, 

 vacuolated in the upper third of the cell, densely aggregated 

 below. 



Nuclear plasm unaltered in arrangement, but staining dark 

 purple or red like the cytoplasm surrounding the nucleus. 



Chromosomes as in controls. 



Nucleolus staining somewhat less brightly. 



Peptones (figs. 4 — 9). — The experiments were made on a 

 large number of leaves, which were fed and fixed on their 

 native moor. It is important to note this, as plants which 

 were taken home did not show the characteristic precipitates 

 about to be described, but only vacuolation. 



Histological Changes. — In all cases the peptone ap- 

 peared as greenish-blue droplets adhering to the exterior 

 surface of the gland cells. In the cells of tliat layer which 

 in my former paper I called tiie third layer of gland cells, 

 the cell sap is particularly rich in tannin, and here is formed 

 a heavy greenish-blue precipitate encrusting the peripheral 

 walls. When viewed with a low power the dark blue line 

 thus formed gives a perfectly characteristic appearance to all 



